Protocols shared by authors via RaP service
Journal
Genetic manipulation of S. aureus mutants
Authors: Katarzyna Wacnik and Simon J Foster
Date: Oct 27, 2020，Views：2370
Original protocol used in research article：
Molecular coordination of Staphylococcus aureus cell division
eLife, Feb 21, 2018; DOI: 10.7554/eLife.32057
LC3 lipidation, membrane fractionation, immunodepletion, and immunoblot
Author: Liang Ge
Date: Oct 26, 2020，Views：1068
Original protocol used in research article：
Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment
eLife, Nov 28, 2014; DOI: 10.7554/eLife.04135
Animal care and stress paradigm
Author: Jaideep Singh Bains
Date: Oct 26, 2020，Views：1154
Original protocol used in research article：
Sexually dimorphic neuronal responses to social isolation
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18726
Immunostaining, hair cell and synaptic counts
Author: Guoqiang Wan
Date: Oct 26, 2020，Views：947
Original protocol used in research article：
Neurotrophin-3 regulates ribbon synapse density in the cochlea and induces synapse regeneration after acoustic trauma
eLife, Oct 20, 2014; DOI: 10.7554/eLife.03564
Assessment of CpG methylation status by bisulfite sequencing
Author: Yijing Su
Date: Oct 23, 2020，Views：899
Original protocol used in research article：
DNA methylation presents distinct binding sites for human transcription factors
eLife, Sep 3, 2013; DOI: 10.7554/eLife.00726
Brain tissue preparation and immunohistochemistry
Authors: Renato Socodato, Teresa Summavielle and João B. Relvas
Date: Oct 23, 2020，Views：948
Original protocol used in research article：
Daily alcohol intake triggers aberrant synaptic pruning leading to synapse loss and anxiety-like behavior
Science Signaling, Sep 21, 2020; DOI: 10.1126/scisignal.aba5754
Measurement of root growth and cell death area in the root tip using Arabidopsis seedlings that grow under DNA stress
Authors: Naoki Takahashi and Masaaki Umeda
Date: Oct 23, 2020，Views：1074
Original protocol used in research article：
A regulatory module controlling stress-induced cell cycle arrest in Arabidopsis
eLife, Apr 4, 2019; DOI: 10.7554/eLife.43944
Fluorescamine-based assay
Authors: Yiwei Yang, Falin Tian, Di Nie, Yuan Liu, Kun Qian, Miaorong Yu, Aohua Wang, Yaqi Zhang, Xinghua Shi and Yong Gan
Date: Oct 22, 2020，Views：846
Original protocol used in research article：
Rapid transport of germ-mimetic nanoparticles with dual conformational polyethylene glycol chains in biological tissues
Science Advances, Feb 6, 2020; DOI: 10.1126/sciadv.aay9937
Dose response curve fitting
Author: Ramnik J Xavier
Date: Oct 21, 2020，Views：1039
Original protocol used in research article：
The kinase DYRK1A reciprocally regulates the differentiation of Th17 and regulatory T cells
eLife, May 22, 2015; DOI: 10.7554/eLife.05920
Cloning and purification of VapB22, VapC22, and VapBC22
Authors: Krishan Gopal Thakur and Ramandeep Singh
Date: Oct 21, 2020，Views：865
Original protocol used in research article：
VapBC22 toxin-antitoxin system from Mycobacterium tuberculosis is required for pathogenesis and modulation of host immune response
Science Advances, Jun 2, 2020; DOI: 10.1126/sciadv.aba6944
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