Protocols shared by authors via RaP service
Journal
Optogenetic stimulation
Author: Shi-Bin Li
Date: Nov 7, 2020，Views：958
Original protocol used in research article：
Hypothalamic circuitry underlying stress-induced insomnia and peripheral immunosuppression
Science Advances, Sep 9, 2020; DOI: 10.1126/sciadv.abc2590
Chromatin immunoprecipitation (ChIP) protocol for the sponge Amphimedon queenslandica
Authors: Federico Gaiti, Bernard M Degnan and Miloš Tanurdžić
Date: Nov 7, 2020，Views：880
Original protocol used in research article：
Landscape of histone modifications in a sponge reveals the origin of animal cis-regulatory complexity
eLife, Apr 11, 2017; DOI: 10.7554/eLife.22194
GeneWeld: a method for efficient targeted integration directed by short homology
Authors: Maura McGrail and Jeffrey Essner
Date: Nov 7, 2020，Views：1552
Original protocol used in research article：
Efficient targeted integration directed by short homology in zebrafish and mammalian cells
eLife, May 15, 2020; DOI: 10.7554/eLife.53968
Splicing efficiency and poly(A) tail length/site
Authors: Daniel R Larson and Murali Palangat
Date: Nov 7, 2020，Views：938
Original protocol used in research article：
Kinetic competition during the transcription cycle results in stochastic RNA processing
eLife, Oct 1, 2014; DOI: 10.7554/eLife.03939
T1RESS sequence design and scan parameters
Author: Robert R Edelman
Date: Nov 6, 2020，Views：857
Original protocol used in research article：
Twofold improved tumor-to-brain contrast using a novel T1 relaxation-enhanced steady-state (T1RESS) MRI technique
Science Advances, Oct 28, 2020; DOI: 10.1126/sciadv.abd1635
Nanogel preparation and characterization
Author: Xiao Xu
Date: Nov 6, 2020，Views：939
Original protocol used in research article：
Self-regulated hirudin delivery for anticoagulant therapy
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abc0382
Crispr-CAS9 knockout of Piezo1
Author: Josefina Ines del Marmol
Date: Nov 5, 2020，Views：964
Original protocol used in research article：
Piezo1 forms a slowly-inactivating mechanosensory channel in mouse embryonic stem cells
eLife, Aug 22, 2018; DOI: 10.7554/eLife.33149
Assay protocols
Authors: Ali H. Alghamdi and Harry P. De Koning
Date: Nov 5, 2020，Views：1121
Original protocol used in research article：
Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416
Construction of the BiG Mito-Split-GFP strain
Authors: Roza Kucharczyk and Hubert Dominique Becker
Date: Nov 5, 2020，Views：1144
Original protocol used in research article：
Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP
eLife, Jul 13, 2020; DOI: 10.7554/eLife.56649
Single cell library preparation
Author: Christopher A Jackson
Date: Nov 5, 2020，Views：1016
Original protocol used in research article：
Gene regulatory network reconstruction using single-cell RNA sequencing of barcoded genotypes in diverse environments
eLife, Jan 27, 2020; DOI: 10.7554/eLife.51254
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.