Protocols shared by authors via RaP service
Journal
Ex vivo live imaging
Authors: Anamika Sharma and Gaiti Hasan
Date: Dec 9, 2020，Views：1170
Original protocol used in research article：
Modulation of flight and feeding behaviours requires presynaptic IP3Rs in dopaminergic neurons
eLife, Nov 6, 2020; DOI: 10.7554/eLife.62297
Bacterial flow cytometry (BUGFlow)
Author: Anne-Katrin Pröbstel
Date: Dec 9, 2020，Views：1145
Original protocol used in research article：
Gut microbiota–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191
16S rRNA gene analysis
Author: Xiaoyuan Zhou
Date: Dec 9, 2020，Views：907
Original protocol used in research article：
Gut microbiota–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191
LNP preparation
Authors: Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2357
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
LNP transfection
Authors: Dan Peer, Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2726
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
Phenotyping of adoptively transferred and endogenous tumor-infiltrating CD8+ T cells in the B16F10 orthotopic melanoma model
Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Dec 8, 2020，Views：902
Original protocol used in research article：
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667
Flow cytometry dissociation protocol for Individual Drosophila brains
Author: Shyama Nandakumar
Date: Dec 7, 2020，Views：1084
Original protocol used in research article：
Polyploidy in the adult Drosophila brain
eLife, Aug 25, 2020; DOI: 10.7554/eLife.54385
Evaluating T cell escape in epitopes previously defined as reactive in clade B consensus mapping - Protocol for Elispot, Peptide handling
Authors: Joanna A Warren and Nilu Goonetilleke
Date: Dec 7, 2020，Views：1030
Original protocol used in research article：
The HIV-1 latent reservoir is largely sensitive to circulating T cells
eLife, Oct 6, 2020; DOI: 10.7554/eLife.57246
Transfection, pool maintenance, and drug addition
Authors: Raghuvir Viswanatha and Norbert Perrimon
Date: Dec 7, 2020，Views：1011
Original protocol used in research article：
Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells
eLife, Jul 27, 2018; DOI: 10.7554/eLife.36333
Stimuli
Author: Paolo Ossola
Date: Dec 7, 2020，Views：931
Original protocol used in research article：
Belief updating in bipolar disorder predicts time of recurrence
eLife, Nov 10, 2020; DOI: 10.7554/eLife.58891
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