Protocols shared by authors via RaP service
Journal
Detergent-resistant membrane isolation and cholesterol measurement
Authors: Ayaka Ito, Xin Rong and Peter Tontonoz
Date: Dec 16, 2020，Views：891
Original protocol used in research article：
LXRs link metabolism to inflammation through Abca1-dependent regulation of membrane composition and TLR signaling
eLife, Jul 14, 2015; DOI: 10.7554/eLife.08009
scRNA-seq analysis
Author: Mikael Simons
Date: Dec 16, 2020，Views：1009
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
Exosome preparation
Author: Hidemasa Oh
Date: Dec 15, 2020，Views：898
Original protocol used in research article：
Cardiosphere-derived exosomal microRNAs for myocardial repair in pediatric dilated cardiomyopathy
Science Translational Medicine, Dec 8, 2020; DOI: 10.1126/scitranslmed.abb3336
Detail Immunostaining Protocol for Pax6 mouse eye samples
Author: Ali R. Djalilian
Date: Dec 15, 2020，Views：795
Original protocol used in research article：
Gene dosage manipulation alleviates manifestations of hereditary PAX6 haploinsufficiency in mice
Science Translational Medicine, Dec 9, 2020; DOI: 10.1126/scitranslmed.aaz4894
Cell culture and reagents
Author: Melanie Girard
Date: Dec 15, 2020，Views：6556
Original protocol used in research article：
Type I interferons drive the maturation of human DC3s with a distinct costimulatory profile characterized by high GITRL
Science Immunology, Nov 12, 2020; DOI: 10.1126/sciimmunol.abe0347
Identification of species-specific expressed genes
Author: Jie Wang
Date: Dec 12, 2020，Views：6698
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
Pre-processing of fiber photometry signal
Author: Richard B Crouse
Date: Dec 11, 2020，Views：1070
Original protocol used in research article：
Acetylcholine is released in the basolateral amygdala in response to predictors of reward and enhances the learning of cue-reward contingency
eLife, Sep 18, 2020; DOI: 10.7554/eLife.57335
RNA constructs and in vitro transcription.
Author: Katharina Reinhard
Date: Dec 10, 2020，Views：1117
Original protocol used in research article：
An RNA vaccine drives expansion and efficacy of claudin-CAR-T cells against solid tumors
Science, Jan 1, 2020; DOI: 10.1126/science.aay5967
STED imaging
Author: Monica M Sousa
Date: Dec 10, 2020，Views：908
Original protocol used in research article：
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction
eLife, Mar 20, 2020; DOI: 10.7554/eLife.55471
Composite agarose-polyacrylamide gel electrophoresis (Composite AgPAGE)
Authors: Kirk Bergstrom, Xindi Shan and Lijun Xia
Date: Dec 9, 2020，Views：1070
Original protocol used in research article：
Proximal colon–derived O-glycosylated mucus encapsulates and modulates the microbiota
Science, Oct 23, 2020; DOI: 10.1126/science.aay7367
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