Protocols shared by authors via RaP service
Journal
Luciferase reporter assay
Authors: Takaki Asano, Bertrand Boisson and Jean-Laurent Casanova
Date: May 7, 2024，Views：576
Original protocol used in research article：
X-linked recessive TLR7 deficiency in ~1% of men under 60 years old with life-threatening COVID-19
Science Immunology, Aug 19, 2021; DOI: 10.1126/sciimmunol.abl4348
Brain virus injection and optic fiber implantation for unraveling M1 layer-specific pathways for pain relief
Authors: Zheng Gan and Rohini Kuner
Date: May 5, 2024，Views：647
Original protocol used in research article：
Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391
Construction of an E. coli strain (W3110) harboring chromosomal 3xFLAG-tagged RutR
Authors: Shun Tu and Sheng-Ce Tao
Date: May 2, 2024，Views：584
Original protocol used in research article：
YcgC represents a new protein deacetylase family in prokaryotes
eLife, Dec 30, 2015; DOI: 10.7554/eLife.05322
Control participants in the online frame rating task
Authors: Efe Soyman, Christian Keysers and Valeria Gazzola
Date: May 1, 2024，Views：53
Original protocol used in research article：
Intracranial human recordings reveal association between neural activity and perceived intensity for the pain of others in the insula
eLife, Nov 3, 2022; DOI: 10.7554/eLife.75197
B cell magnetic fractionation
Authors: Silvia Portugal and Peter D Crompton
Date: May 1, 2024，Views：30
Original protocol used in research article：
Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function
eLife, May 8, 2015; DOI: 10.7554/eLife.07218
Protocol for Determining RNA Localization by Subcellular RNA Fractionation
Authors: Irfana Saleem, Bhopal Mohapatra, Hamid Band and Vimla Band
Date: Apr 22, 2024，Views：829
Original protocol used in research article：
The Mammalian Ecdysoneless Protein Interacts with RNA Helicase DDX39A To Regulate Nuclear mRNA Export
Molecular and Cellular Biology, Jun 23, 2021; DOI: 10.1128/mcb.00103-21
In vivo experiments in humanized Alb−/−FcRn−/−hFcRn+/+ mice
Author: Joan Clària
Date: Apr 22, 2024，Views：18
Original protocol used in research article：
Albumin internalizes and inhibits endosomal TLR signaling in leukocytes from patients with decompensated cirrhosis
Science Translational Medicine, Oct 20, 2020; DOI: 10.1126/scitranslmed.aax5135
In-gel fluorescence imaging and immunoblotting
Authors: Hayashi Yamamoto and Noboru Mizushima
Date: Apr 19, 2024，Views：908
Original protocol used in research article：
A pulse-chasable reporter processing assay for mammalian autophagic flux with HaloTag
eLife, ; DOI: 10.7554/eLife.78923
Lead contact
Authors: Wen Fang and Liping Wang
Date: Apr 18, 2024，Views：110
Original protocol used in research article：
Neural dynamics of causal inference in the macaque frontoparietal circuit
eLife, Oct 24, 2022; DOI: 10.7554/eLife.76145
Cryo-Sectioning of viral tracer-labeled macaque brains
Authors: Mingchao Yan and Zheng Wang
Date: Apr 15, 2024，Views：823
Original protocol used in research article：
Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution and comparison with diffusion tractography
eLife, ; DOI: 10.7554/eLife.72534
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