Protocols shared by authors via RaP service
Journal
Preparation of libraries for RNA-seq
Authors: Jamie R Wangen and Rachel Green
Date: Apr 9, 2021，Views：839
Original protocol used in research article：
Stop codon context influences genome-wide stimulation of termination codon readthrough by aminoglycosides
eLife, Jan 23, 2020; DOI: 10.7554/eLife.52611
RRTS calculation
Authors: Jamie Wangen and Rachel Green
Date: Apr 9, 2021，Views：900
Original protocol used in research article：
Stop codon context influences genome-wide stimulation of termination codon readthrough by aminoglycosides
eLife, Jan 23, 2020; DOI: 10.7554/eLife.52611
Bone sectioning, immunostaining, and confocal imaging
Authors: Lei Ding and Sean J Morrison
Date: Apr 9, 2021，Views：891
Original protocol used in research article：
Hematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1
eLife, Mar 30, 2015; DOI: 10.7554/eLife.05521
Statistical analysis
Authors: Thanaphong Phongpreecha and Thomas J. Montine
Date: Apr 8, 2021，Views：856
Original protocol used in research article：
Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575
Contribution of inter-relatedness chimpanzees to differential expression analysis
Author: Benjamin Jung Fair
Date: Apr 8, 2021，Views：847
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
Single-nucleus RNA-seq data generation
Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 7, 2021，Views：1037
Original protocol used in research article：
Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522
Microtubule co-sedimentation from worm extract
Author: Shaohe Wang
Date: Apr 7, 2021，Views：857
Original protocol used in research article：
NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08649
Extending 3' UTRs in the transcriptome index
Authors: Lauren M Saunders, Cole Trapnell and David M Parichy
Date: Apr 7, 2021，Views：1005
Original protocol used in research article：
Thyroid hormone regulates distinct paths to maturation in pigment cell lineages
eLife, May 29, 2019; DOI: 10.7554/eLife.45181
Cell transformation assay
Authors: Karoliina Stefanius and Kim Orth
Date: Apr 7, 2021，Views：989
Original protocol used in research article：
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
In situ hybridization
Authors: Kaiwen Kam and Carolina Thörn Perez
Date: Apr 7, 2021，Views：902
Original protocol used in research article：
Opioids modulate an emergent rhythmogenic process to depress breathing
eLife, Dec 16, 2019; DOI: 10.7554/eLife.50613
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.