Protocols shared by authors via RaP service
Journal
Astrocyte primary cultures
Authors: Álvaro Herrero-Navarro and Guillermina López-Bendito
Date: Apr 22, 2021，Views：864
Original protocol used in research article：
Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming
Science Advances, Apr 7, 2021; DOI: 10.1126/sciadv.abe8978
Liposome dye release assay
Authors: Gemma E Swinscoe and Stephen Griffin
Date: Apr 21, 2021，Views：1021
Original protocol used in research article：
Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy
eLife, Nov 10, 2020; DOI: 10.7554/eLife.52555
Biotinylation of Smc3p-AviTag reporters
Authors: Siheng Xiang and Douglas Koshland
Date: Apr 21, 2021，Views：914
Original protocol used in research article：
Cohesin architecture and clustering in vivo
eLife, Feb 17, 2021; DOI: 10.7554/eLife.62243
Chromatin Immunoprecipitation, sequencing and data analysis in Drosophila S2 cells
Authors: Anand K Singh, Jie Zhang, Daniel Hebenstreit and Saverio Brogna
Date: Apr 20, 2021，Views：1484
Original protocol used in research article：
Evidence of slightly increased Pol II pausing in UPF1-depleted Drosophila melanogaster cells
microPublication Biology, Oct 16, 2020; DOI: 10.17912/micropub.biology.000319
Single-nucleus ATAC-seq data generation
Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 19, 2021，Views：1031
Original protocol used in research article：
Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522
LNP quantification and encapsulation
Author: Dan Peer
Date: Apr 19, 2021，Views：1106
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
Flow cytometric analysis (FACS)
Authors: Andy Peng Xiang and Zhengqi Lu
Date: Apr 18, 2021，Views：826
Original protocol used in research article：
Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abe4827
Purification of cells by FACS
Authors: Hsin-I Jen and Andrew K Groves
Date: Apr 16, 2021，Views：861
Original protocol used in research article：
Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1
eLife, Apr 29, 2019; DOI: 10.7554/eLife.44328
RNA-seq
Authors: Hsin-I Jen and Andrew K Groves
Date: Apr 16, 2021，Views：877
Original protocol used in research article：
Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1
eLife, Apr 29, 2019; DOI: 10.7554/eLife.44328
Expression, purification and on-bead reconstitution of MsbA and MscS into peptidiscs
Authors: Gabriella Angiulli, Franck Duong Van Hoa and Thomas Walz
Date: Apr 16, 2021，Views：1013
Original protocol used in research article：
New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins
eLife, Mar 3, 2020; DOI: 10.7554/eLife.53530
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