Protocols shared by authors via RaP service
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ER and mitochondrial ATP level analysis by flow cytometry
Author: Jing Yong
Date: Apr 23, 2021，Views：1223
Original protocol used in research article：
Mitochondria supply ATP to the ER through a mechanism antagonized by cytosolic Ca2+
eLife, Sep 9, 2019; DOI: 10.7554/eLife.49682
Multivariate analyses of shape
Authors: V. B. Baliga and D. L. Altshuler
Date: Apr 23, 2021，Views：897
Original protocol used in research article：
Range of motion in the avian wing is strongly associated with flight behavior and body mass
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6670
RNAscope in situ hybridization
Authors: Alexander T Chesler and Claire E Le Pichon
Date: Apr 23, 2021，Views：894
Original protocol used in research article：
Dual leucine zipper kinase is required for mechanical allodynia and microgliosis after nerve injury
eLife, Jul 3, 2018; DOI: 10.7554/eLife.33910
Characterizing the pressure sensor
Authors: Yoonseok Park and John A. Rogers
Date: Apr 23, 2021，Views：968
Original protocol used in research article：
Wireless, skin-interfaced sensors for compression therapy
Science Advances, Dec 4, 2020; DOI: 10.1126/sciadv.abe1655
Immunohistochemistry and synaptic puncta analysis
Authors: W Christopher Risher and Cagla Eroglu
Date: Apr 23, 2021，Views：946
Original protocol used in research article：
Astrocytes refine cortical connectivity at dendritic spines
eLife, Dec 17, 2014; DOI: 10.7554/eLife.04047
Astrocyte primary cultures
Authors: Álvaro Herrero-Navarro and Guillermina López-Bendito
Date: Apr 22, 2021，Views：864
Original protocol used in research article：
Astrocytes and neurons share region-specific transcriptional signatures that confer regional identity to neuronal reprogramming
Science Advances, Apr 7, 2021; DOI: 10.1126/sciadv.abe8978
Liposome dye release assay
Authors: Gemma E Swinscoe and Stephen Griffin
Date: Apr 21, 2021，Views：1016
Original protocol used in research article：
Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy
eLife, Nov 10, 2020; DOI: 10.7554/eLife.52555
Biotinylation of Smc3p-AviTag reporters
Authors: Siheng Xiang and Douglas Koshland
Date: Apr 21, 2021，Views：913
Original protocol used in research article：
Cohesin architecture and clustering in vivo
eLife, Feb 17, 2021; DOI: 10.7554/eLife.62243
Chromatin Immunoprecipitation, sequencing and data analysis in Drosophila S2 cells
Authors: Anand K Singh, Jie Zhang, Daniel Hebenstreit and Saverio Brogna
Date: Apr 20, 2021，Views：1482
Original protocol used in research article：
Evidence of slightly increased Pol II pausing in UPF1-depleted Drosophila melanogaster cells
microPublication Biology, Oct 16, 2020; DOI: 10.17912/micropub.biology.000319
Single-nucleus ATAC-seq data generation
Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 19, 2021，Views：1030
Original protocol used in research article：
Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522
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