Protocols shared by authors via RaP service
Journal
Parameter optimizations of the three machine learning algorithm models
Authors: Ni Huang and Li Wang
Date: May 17, 2021，Views：875
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Whole Mount in situ Hybridization (WISH) on mouse embryos
Authors: Matthew J Anderson and Mark Lewandoski
Date: May 17, 2021，Views：860
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
Characterization of CAR-transduced T cell cytotoxic potential
Authors: Vanaja Konduri and William K. Decker
Date: May 17, 2021，Views：906
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
Immunofluorescence, Sirius red staining, and intensity quantitations
Authors: Denise Gay, Paul-Henri Romeo and Denise Gay
Date: May 16, 2021，Views：927
Original protocol used in research article：
Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aay3704
T cell migration assays
Author: Dong Wang
Date: May 16, 2021，Views：979
Original protocol used in research article：
Tumor immunological phenotype signature-based high-throughput screening for the discovery of combination immunotherapy compounds
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abd7851
Identification of gene loss
Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：929
Original protocol used in research article：
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459
Gene family expansion and contraction
Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：953
Original protocol used in research article：
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459
Immunofluorescence staining of embryos
Author: W. S. B. Yeung
Date: May 14, 2021，Views：880
Original protocol used in research article：
Let-7 derived from endometrial extracellular vesicles is an important inducer of embryonic diapause in mice
Science Advances, Sep 9, 2020; DOI: 10.1126/sciadv.aaz7070
Rarefaction analysis
Authors: Daniel A Russell and Graham F Hatfull
Date: May 13, 2021，Views：908
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
Phages and genomes
Authors: Daniel A Russell and Graham F Hatfull
Date: May 13, 2021，Views：949
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.