Protocols shared by authors via RaP service
Journal
Neural induction
Authors: G. Zhao and J. Fu
Date: Apr 26, 2021，Views：918
Original protocol used in research article：
Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI: 10.1126/sciadv.aax5933
Fabrication of gel beds
Authors: G. Zhao and J. Fu
Date: Apr 26, 2021，Views：804
Original protocol used in research article：
Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI: 10.1126/sciadv.aax5933
Confocal microscopy and analysis
Authors: Maria D Purice, Sean D Speese and Mary A Logan
Date: Apr 26, 2021，Views：1428
Original protocol used in research article：
A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade
eLife, Aug 21, 2017; DOI: 10.7554/eLife.23611
CNV detection
Authors: Jean-Laurent Casanova and Qian Zhang
Date: Apr 26, 2021，Views：927
Original protocol used in research article：
Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570
Influenza virus protein
Authors: Jean-Laurent Casanova and Qian Zhang
Date: Apr 26, 2021，Views：1029
Original protocol used in research article：
Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570
Patients
Authors: Qian Zhang and Jean-Laurent Casanova
Date: Apr 26, 2021，Views：1023
Original protocol used in research article：
Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570
Mouse brain stereotaxic injections
Authors: Huda Y. Zoghbi, Yingyao Shao and Yehezkel Sztainberg
Date: Apr 26, 2021，Views：2975
Original protocol used in research article：
Antisense oligonucleotide therapy in a humanized mouse model of MECP2 duplication syndrome
Science Translational Medicine, Mar 2, 2021; DOI: 10.1126/scitranslmed.aaz7785
Trapping of photocycle intermediates
Authors: Orawan Jakdetchai, Johanna Becker-Baldus, Josef Wachtveitl and Clemens Glaubitz
Date: Apr 26, 2021，Views：862
Original protocol used in research article：
Probing the photointermediates of light-driven sodium ion pump KR2 by DNP-enhanced solid-state NMR
Science Advances, Mar 12, 2021; DOI: 10.1126/sciadv.abf4213
Cell preparation and scRNA-seq
Authors: Yiran Hou, Guoyan Zhao and Ting Wang
Date: Apr 25, 2021，Views：1009
Original protocol used in research article：
Cellular diversity of the regenerating caudal fin
Science Advances, Aug 12, 2020; DOI: 10.1126/sciadv.aba2084
Quantification of ER ATP and Ca2+ levels by fluorescence ratiometry analysis in live cells
Author: Jing Yong
Date: Apr 23, 2021，Views：918
Original protocol used in research article：
Mitochondria supply ATP to the ER through a mechanism antagonized by cytosolic Ca2+
eLife, Sep 9, 2019; DOI: 10.7554/eLife.49682
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