Protocols shared by authors via RaP service
Journal
Immunoblotting
Authors: Benjamin Demarco, Kaiwen Chen and Petr Broz
Date: May 10, 2021，Views：876
Original protocol used in research article：
Caspase-8–dependent gasdermin D cleavage promotes antimicrobial defense but confers susceptibility to TNF-induced lethality
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc3465
Gene-specific m6A qPCR
Authors: Chris Bass and Youjun Zhang
Date: May 9, 2021，Views：1262
Original protocol used in research article：
Epitranscriptomic regulation of insecticide resistance
Science Advances, May 5, 2021; DOI: 10.1126/sciadv.abe5903
Statistical analysis
Authors: David Chen, Josephine Wu and Gordana Vunjak-Novakovic
Date: May 9, 2021，Views：875
Original protocol used in research article：
Tissue engineered autologous cartilage-bone grafts for temporomandibular joint regeneration
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.abb6683
3-D printing of window and clip
Authors: Qiang Huang, Rudolf Jaenisch and Xiling Shen
Date: May 7, 2021，Views：897
Original protocol used in research article：
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI: 10.1126/science.aba0210
Sperm penetration and binding assays
Authors: Ismael Lamas-Toranzo, María Jiménez-Movilla and Pablo Bermejo-Álvarez
Date: May 7, 2021，Views：844
Original protocol used in research article：
TMEM95 is a sperm membrane protein essential for mammalian fertilization
eLife, Jun 2, 2020; DOI: 10.7554/eLife.53913
Isolation and phospholipid enrichment of muscle mitochondria and mitoplasts
Authors: Alexandre Prola, Aymeline Vandestienne, Frédéric Joubert, Laurent Tiret and Fanny Pilot-Storck
Date: May 7, 2021，Views：1140
Original protocol used in research article：
Cardiolipin content controls mitochondrial coupling and energetic efficiency in muscle
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd6322
FUCCI live-cell imaging
Author: Andrew Burgess
Date: May 6, 2021，Views：955
Original protocol used in research article：
Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma
eLife, Jun 9, 2020; DOI: 10.7554/eLife.53367
PhosFate analysis
Authors: Jonathan M Budzik and Jeffery S Cox
Date: May 6, 2021，Views：904
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
Web app
Authors: Jonathan M Budzik and Jeffery S Cox
Date: May 6, 2021，Views：906
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
RNAseq
Authors: Jonathan M Budzik and Jeffery S Cox
Date: May 6, 2021，Views：832
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
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