Protocols shared by authors via RaP service
Journal
Coexpression and cis-motif enrichment analyses
Authors: Sandip M Kale and Ravi Koppolu
Date: May 22, 2021，Views：1011
Original protocol used in research article：
Transcriptional landscapes of floral meristems in barley
Science Advances, Apr 28, 2021; DOI: 10.1126/sciadv.abf0832
Immunofluorescence
Authors: Li-Yin Hung and De’Broski R. Herbert
Date: May 21, 2021，Views：954
Original protocol used in research article：
Cellular context of IL-33 expression dictates impact on anti-helminth immunity
Science Immunology, Nov 12, 2020; DOI: 10.1126/sciimmunol.abc6259
Preparation of yeast membranes
Authors: Sisi Lin, Subhajit Mukherjee, Juanjuan Li, Weiliang Hou, Chao Pan and Jinyao Liu
Date: May 21, 2021，Views：1039
Original protocol used in research article：
Mucosal immunity–mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches
Science Advances, May 12, 2021; DOI: 10.1126/sciadv.abf0677
MCAO Stroke Model Protocol for Mouse
Authors: Luke R Lemmerman, Hallie N Harris, W. David Arnold, Natalia Higuita-Castro and Daniel Gallego-Perez
Date: May 21, 2021，Views：1485
Original protocol used in research article：
Nanotransfection-based vasculogenic cell reprogramming drives functional recovery in a mouse model of ischemic stroke
Science Advances, Mar 18, 2021; DOI: 10.1126/sciadv.abd4735
Cell lines
Authors: Zuodong Zhao, Zhuqiang Zhang and Bing Zhu
Date: May 20, 2021，Views：823
Original protocol used in research article：
Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness
eLife, Nov 6, 2020; DOI: 10.7554/eLife.61965
Intestinal organoid cultures
Authors: Zuri Sullivan and Ruslan Medzhitov
Date: May 18, 2021，Views：909
Original protocol used in research article：
γδ T cells regulate the intestinal response to nutrient sensing
Science, Mar 19, 2021; DOI: 10.1126/science.aba8310
Data filtering and genome assembly
Authors: Reuben W Nowell and Timothy G Barraclough
Date: May 18, 2021，Views：896
Original protocol used in research article：
Evolutionary dynamics of transposable elements in bdelloid rotifers
eLife, Feb 5, 2021; DOI: 10.7554/eLife.63194
Data
Authors: Li Wang and Ni Huang
Date: May 17, 2021，Views：971
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Parameter optimizations of the three machine learning algorithm models
Authors: Ni Huang and Li Wang
Date: May 17, 2021，Views：886
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Whole Mount in situ Hybridization (WISH) on mouse embryos
Authors: Matthew J Anderson and Mark Lewandoski
Date: May 17, 2021，Views：904
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.