Protocols shared by authors via RaP service
Journal
T cell migration assays
Author: Dong Wang
Date: May 16, 2021，Views：980
Original protocol used in research article：
Tumor immunological phenotype signature-based high-throughput screening for the discovery of combination immunotherapy compounds
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abd7851
Identification of gene loss
Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：930
Original protocol used in research article：
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459
Gene family expansion and contraction
Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：955
Original protocol used in research article：
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459
Immunofluorescence staining of embryos
Author: W. S. B. Yeung
Date: May 14, 2021，Views：883
Original protocol used in research article：
Let-7 derived from endometrial extracellular vesicles is an important inducer of embryonic diapause in mice
Science Advances, Sep 9, 2020; DOI: 10.1126/sciadv.aaz7070
Rarefaction analysis
Authors: Daniel A Russell and Graham F Hatfull
Date: May 13, 2021，Views：912
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
Phages and genomes
Authors: Daniel A Russell and Graham F Hatfull
Date: May 13, 2021，Views：958
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
ASC protein aggregates (specks)
Author: Michael F McDermott
Date: May 13, 2021，Views：957
Original protocol used in research article：
ENaC-mediated sodium influx exacerbates NLRP3-dependent inflammation in cystic fibrosis
eLife, Sep 18, 2019; DOI: 10.7554/eLife.49248
Surgical procedure
Authors: Qiang Huang, Rudolf Jaenisch and Xiling Shen
Date: May 12, 2021，Views：923
Original protocol used in research article：
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI: 10.1126/science.aba0210
Microscopy and live-cell imaging
Author: Perunthottathu K Umasankar
Date: May 12, 2021，Views：942
Original protocol used in research article：
A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing
eLife, Oct 10, 2014; DOI: 10.7554/eLife.04137
Cell culture, transfections and immunofluorescence
Author: Perunthottathu K Umasankar
Date: May 12, 2021，Views：1439
Original protocol used in research article：
A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing
eLife, Oct 10, 2014; DOI: 10.7554/eLife.04137
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