Protocols shared by authors via RaP service
Journal
Remodeling of the spleen tissue
Authors: Lintao Wang, Junfeng Zhang, Chunming Wang and Lei Dong
Date: May 31, 2021，Views：890
Original protocol used in research article：
Transforming the spleen into a liver-like organ in vivo
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz9974
Purification of DDB1ΔBPB
Author: Longzhi Cao
Date: May 31, 2021，Views：1005
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Metabolomic analysis
Authors: Shenglin Luo, Yang Wang and Chunmeng Shi
Date: May 30, 2021，Views：944
Original protocol used in research article：
Near-infrared oxidative phosphorylation inhibitor integrates acute myeloid leukemia–targeted imaging and therapy
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abb6104
IFNγ ELISpot
Author: Christopher M. Haqq
Date: May 27, 2021，Views：1009
Original protocol used in research article：
A lymph node–targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2
Science Advances, Feb 4, 2021; DOI: 10.1126/sciadv.abe5819
Single hepatocyte collection
Authors: S. Sun and J. Vijg
Date: May 26, 2021，Views：945
Original protocol used in research article：
Single-cell analysis reveals different age-related somatic mutation profiles between stem and differentiated cells in human liver
Science Advances, Jan 30, 2020; DOI: 10.1126/sciadv.aax2659
Quantification of food intake
Authors: Caroline Lei Wee, Sam Kunes and Erin Song
Date: May 26, 2021，Views：1125
Original protocol used in research article：
A bidirectional network for appetite control in larval zebrafish
eLife, Oct 18, 2019; DOI: 10.7554/eLife.43775
Whole-mount immunostaining
Authors: Caroline Lei Wee, Sam Kunes and Erin Song
Date: May 26, 2021，Views：1059
Original protocol used in research article：
A bidirectional network for appetite control in larval zebrafish
eLife, Oct 18, 2019; DOI: 10.7554/eLife.43775
Fecundity assays
Authors: Brooke Zanco and Matthew DW Piper
Date: May 26, 2021，Views：1645
Original protocol used in research article：
A dietary sterol trade-off determines lifespan responses to dietary restriction in Drosophila melanogaster females
eLife, Jan 26, 2021; DOI: 10.7554/eLife.62335
scRNA/TCR-seq data processing
Authors: Paul Gueguen, Joshua J. Waterfall and Sebastian Amigorena
Date: May 26, 2021，Views：986
Original protocol used in research article：
Contribution of resident and circulating precursors to tumor-infiltrating CD8+ T cell populations in lung cancer
Science Immunology, Jan 29, 2021; DOI: 10.1126/sciimmunol.abd5778
Generation of cell lines stably expressing ACVR1-IPF
Authors: Anassuya Ramachandran and Caroline S Hill
Date: May 25, 2021，Views：824
Original protocol used in research article：
TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition
eLife, Jan 29, 2018; DOI: 10.7554/eLife.31756
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