Protocols shared by authors via RaP service
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Immunofluorescence staining – quantification of activated-caspase 3-positive cells in brain slices:
Authors: Oriane Blanquie, Heiko J Luhmann, Jenq-Wei Yang, Werner Kilb, Salim Sharopov and Anne Sinning
Date: Jul 14, 2020，Views：1409
Original protocol used in research article：
Electrical activity controls area-specific expression of neuronal apoptosis in the mouse developing cerebral cortex
eLife, Aug 21, 2017; DOI: 10.7554/eLife.27696
Lung tumor volume measurements from X-ray micro-computed tomography (μCT) scans
Authors: Caroline Contat and Etienne Meylan
Date: Jul 14, 2020，Views：1199
Original protocol used in research article：
Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth
eLife, Jun 23, 2020; DOI: 10.7554/eLife.53618
2-NBDG uptake
Authors: Kenneth Kin Lam Wong and Esther M Verheyen
Date: Jul 13, 2020，Views：1216
Original protocol used in research article：
A positive feedback loop between Myc and aerobic glycolysis sustains tumor growth in a Drosophila tumor model
eLife, Jul 1, 2019; DOI: 10.7554/eLife.46315
Simulation setup and refinement of non-ribosomal systems
Authors: Maxim Igaev, Andrea C Vaiana and Helmut Grubmüller
Date: Jul 13, 2020，Views：1091
Original protocol used in research article：
Automated cryo-EM structure refinement using correlation-driven molecular dynamics
eLife, Mar 4, 2019; DOI: 10.7554/eLife.43542
Functional connectivity analysis
Author: Benjamin Dann
Date: Jul 13, 2020，Views：1070
Original protocol used in research article：
Uniting functional network topology and oscillations in the fronto-parietal single unit network of behaving primates
eLife, Aug 15, 2016; DOI: 10.7554/eLife.15719
Electrophysiology
Authors: Qingqing Liu, Aike Guo and Xing Yang
Date: Jul 13, 2020，Views：1316
Original protocol used in research article：
Gap junction networks in mushroom bodies participate in visual learning and memory in Drosophila
eLife, May 24, 2016; DOI: 10.7554/eLife.13238
Immunohistochemistry
Author: Akihiro Yamanaka
Date: Jul 12, 2020，Views：1016
Original protocol used in research article：
Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice
eLife, Jun 4, 2019; DOI: 10.7554/eLife.44927
Plant material and growth conditions
Author: Susan Duncan
Date: Jul 11, 2020，Views：1032
Original protocol used in research article：
Seasonal shift in timing of vernalization as an adaptation to extreme winter
eLife, Jul 23, 2015; DOI: 10.7554/eLife.06620
Expression analysis
Author: Susan Duncan
Date: Jul 10, 2020，Views：1087
Original protocol used in research article：
Seasonal shift in timing of vernalization as an adaptation to extreme winter
eLife, Jul 23, 2015; DOI: 10.7554/eLife.06620
Copy number variation analysis
Author: Petar Pajic
Date: Jul 10, 2020，Views：1411
Original protocol used in research article：
Independent amylase gene copy number bursts correlate with dietary preferences in mammals
eLife, May 14, 2019; DOI: 10.7554/eLife.44628
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