Protocols shared by authors via RaP service
Journal
O2 release from PCC7942
Author: Huanhuan Chen
Date: Jul 7, 2020，Views：1113
Original protocol used in research article：
Dissolved oxygen from microalgae-gel patch promotes chronic wound healing in diabetes
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aba4311
Viral-mediated gene delivery for in vivo circuit manipulation in neonatal mice
Authors: Yasunobu Murata and Matthew T Colonnese
Date: Jul 6, 2020，Views：1132
Original protocol used in research article：
GABAergic interneurons excite neonatal hippocampus in vivo
Science Advances, Jun 11, 2020; DOI: 10.1126/sciadv.aba1430
Glutaraldehyde Cross-linked Nun/TEC Preparation for CryoEM
Authors: Jin Young Kang and Seth A Darst
Date: Jul 6, 2020，Views：1280
Original protocol used in research article：
Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex
eLife, Mar 20, 2017; DOI: 10.7554/eLife.25478
Glutaraldehyde Cross-linked TEC preparation for CryoEM
Authors: Jin Young Kang and Seth A Darst
Date: Jul 5, 2020，Views：1463
Original protocol used in research article：
Structural basis of transcription arrest by coliphage HK022 Nun in an Escherichia coli RNA polymerase elongation complex
eLife, Mar 20, 2017; DOI: 10.7554/eLife.25478
Implementing CILP
Author: Amanda Lea
Date: Jul 5, 2020，Views：1052
Original protocol used in research article：
Genetic and environmental perturbations lead to regulatory decoherence
eLife, Mar 5, 2019; DOI: 10.7554/eLife.40538
Pulldown assay
Author: Richard T Lee
Date: Jul 5, 2020，Views：1215
Original protocol used in research article：
Diabetes regulates fructose absorption through thioredoxin-interacting protein
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18313
Cell culture, nuclei isolation and GRO-seq library preparation
Authors: Iris Jonkers and John T Lis
Date: Jul 5, 2020，Views：1208
Original protocol used in research article：
Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons
eLife, Apr 29, 2014; DOI: 10.7554/eLife.02407
In vivo antitumor therapy through AUNPs
Author: Duo Mao
Date: Jul 5, 2020，Views：1049
Original protocol used in research article：
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712
Human pluripotent stem cell culture and neural differentiation
Authors: Yan Liu and Fang Yuan
Date: Jul 4, 2020，Views：1142
Original protocol used in research article：
Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6
eLife, Sep 25, 2018; DOI: 10.7554/eLife.37382
Preparation of cell samples with relatively uniform distribution of Alexa-647
Author: Yanxiang Ni
Date: Jul 3, 2020，Views：1045
Original protocol used in research article：
Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes
eLife, May 9, 2017; DOI: 10.7554/eLife.21660
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