Protocols shared by authors via RaP service
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Fluorescence quenching assay
Author: Mable Lam
Date: Jul 15, 2020，Views：1024
Original protocol used in research article：
Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52291
Peptide pulldown with Fc-tagged ECD proteins
Author: Mable Lam
Date: Jul 14, 2020，Views：1032
Original protocol used in research article：
Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52291
Kcr peptide enrichment
Authors: Zhongyi Cheng and Jing Liang
Date: Jul 14, 2020，Views：1022
Original protocol used in research article：
Global crotonylome reveals CDYL-regulated RPA1 crotonylation in homologous recombination–mediated DNA repair
Science Advances, Mar 12, 2020; DOI: 10.1126/sciadv.aay4697
Lectin staining
Author: Katarina Valoskova
Date: Jul 14, 2020，Views：1427
Original protocol used in research article：
A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion
eLife, Mar 26, 2019; DOI: 10.7554/eLife.41801
Immunofluorescence staining – quantification of activated-caspase 3-positive cells in brain slices:
Authors: Oriane Blanquie, Heiko J Luhmann, Jenq-Wei Yang, Werner Kilb, Salim Sharopov and Anne Sinning
Date: Jul 14, 2020，Views：1397
Original protocol used in research article：
Electrical activity controls area-specific expression of neuronal apoptosis in the mouse developing cerebral cortex
eLife, Aug 21, 2017; DOI: 10.7554/eLife.27696
Lung tumor volume measurements from X-ray micro-computed tomography (μCT) scans
Authors: Caroline Contat and Etienne Meylan
Date: Jul 14, 2020，Views：1197
Original protocol used in research article：
Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth
eLife, Jun 23, 2020; DOI: 10.7554/eLife.53618
2-NBDG uptake
Authors: Kenneth Kin Lam Wong and Esther M Verheyen
Date: Jul 13, 2020，Views：1215
Original protocol used in research article：
A positive feedback loop between Myc and aerobic glycolysis sustains tumor growth in a Drosophila tumor model
eLife, Jul 1, 2019; DOI: 10.7554/eLife.46315
Simulation setup and refinement of non-ribosomal systems
Authors: Maxim Igaev, Andrea C Vaiana and Helmut Grubmüller
Date: Jul 13, 2020，Views：1085
Original protocol used in research article：
Automated cryo-EM structure refinement using correlation-driven molecular dynamics
eLife, Mar 4, 2019; DOI: 10.7554/eLife.43542
Functional connectivity analysis
Author: Benjamin Dann
Date: Jul 13, 2020，Views：1068
Original protocol used in research article：
Uniting functional network topology and oscillations in the fronto-parietal single unit network of behaving primates
eLife, Aug 15, 2016; DOI: 10.7554/eLife.15719
Electrophysiology
Authors: Qingqing Liu, Aike Guo and Xing Yang
Date: Jul 13, 2020，Views：1309
Original protocol used in research article：
Gap junction networks in mushroom bodies participate in visual learning and memory in Drosophila
eLife, May 24, 2016; DOI: 10.7554/eLife.13238
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