Protocols shared by authors via RaP service
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Preparation of yeast membranes
Authors: Sisi Lin, Subhajit Mukherjee, Juanjuan Li, Weiliang Hou, Chao Pan and Jinyao Liu
Date: May 21, 2021，Views：1011
Original protocol used in research article：
Mucosal immunity–mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches
Science Advances, May 12, 2021; DOI: 10.1126/sciadv.abf0677
MCAO Stroke Model Protocol for Mouse
Authors: Luke R Lemmerman, Hallie N Harris, W. David Arnold, Natalia Higuita-Castro and Daniel Gallego-Perez
Date: May 21, 2021，Views：1356
Original protocol used in research article：
Nanotransfection-based vasculogenic cell reprogramming drives functional recovery in a mouse model of ischemic stroke
Science Advances, Mar 18, 2021; DOI: 10.1126/sciadv.abd4735
Cell lines
Authors: Zuodong Zhao, Zhuqiang Zhang and Bing Zhu
Date: May 20, 2021，Views：804
Original protocol used in research article：
Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness
eLife, Nov 6, 2020; DOI: 10.7554/eLife.61965
Intestinal organoid cultures
Authors: Zuri Sullivan and Ruslan Medzhitov
Date: May 18, 2021，Views：885
Original protocol used in research article：
γδ T cells regulate the intestinal response to nutrient sensing
Science, Mar 19, 2021; DOI: 10.1126/science.aba8310
Data filtering and genome assembly
Authors: Reuben W Nowell and Timothy G Barraclough
Date: May 18, 2021，Views：866
Original protocol used in research article：
Evolutionary dynamics of transposable elements in bdelloid rotifers
eLife, Feb 5, 2021; DOI: 10.7554/eLife.63194
Data
Authors: Li Wang and Ni Huang
Date: May 17, 2021，Views：960
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Parameter optimizations of the three machine learning algorithm models
Authors: Ni Huang and Li Wang
Date: May 17, 2021，Views：875
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Whole Mount in situ Hybridization (WISH) on mouse embryos
Authors: Matthew J Anderson and Mark Lewandoski
Date: May 17, 2021，Views：866
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
Characterization of CAR-transduced T cell cytotoxic potential
Authors: Vanaja Konduri and William K. Decker
Date: May 17, 2021，Views：908
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
Immunofluorescence, Sirius red staining, and intensity quantitations
Authors: Denise Gay, Paul-Henri Romeo and Denise Gay
Date: May 16, 2021，Views：931
Original protocol used in research article：
Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aay3704
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