Protocols shared by authors via RaP service
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Respiration measurements
Author: David C Chan
Date: Dec 8, 2019，Views：1162
Original protocol used in research article：
Mitochondrial fusion is required for spermatogonial differentiation and meiosis
eLife, Oct 9, 2019; DOI: 10.7554/eLife.51601
Drosha cleavage assay
Author: Tsui Han Loo
Date: Dec 6, 2019，Views：1204
Original protocol used in research article：
The mammalian LINC complex component SUN1 regulates muscle regeneration by modulating drosha activity
eLife, Nov 5, 2019; DOI: 10.7554/eLife.49485
Immunofluorescence staining - Vibratome Sections for Oligodendrocyte morphological analysis
Author: Matthew Swire
Date: Dec 6, 2019，Views：10140
Original protocol used in research article：
Endothelin signalling mediates experience-dependent myelination in the CNS
eLife, Oct 28, 2019; DOI: 10.7554/eLife.49493
General Protocol for Resistance Assays in Liquid Media
Author: Nathaniel J Traaseth
Date: Dec 4, 2019，Views：1130
Original protocol used in research article：
Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations
eLife, Oct 22, 2019; DOI: 10.7554/eLife.48909
C2C12 culture and generation of Gene-modified C2C12 cells
Author: Lin Mei
Date: Dec 4, 2019，Views：1197
Original protocol used in research article：
A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome
eLife, Sep 24, 2019; DOI: 10.7554/eLife.49180
Quantification of tracheal density in IFMs
Author: Stefan Luschnig
Date: Dec 4, 2019，Views：1184
Original protocol used in research article：
Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857
Growth chamber experiments
Author: Steven Penfield
Date: Dec 4, 2019，Views：1186
Original protocol used in research article：
Flowering time and seed dormancy control use external coincidence to generate life history strategy
eLife, Mar 31, 2015; DOI: 10.7554/eLife.05557
Isolation of skeletal muscle-derived mononuclear cells
Author: So-ichiro Fukada
Date: Dec 3, 2019，Views：1079
Original protocol used in research article：
Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle
eLife, Sep 23, 2019; DOI: 10.7554/eLife.48284
Purification of mammalian dynein-dynactin
Author: Ahmet Yildiz
Date: Dec 3, 2019，Views：1162
Original protocol used in research article：
Kinesin and dynein use distinct mechanisms to bypass obstacles
eLife, Sep 9, 2019; DOI: 10.7554/eLife.48629
In vitro differentiation of mESCs in embryoid bodies
Author: Lars Schomacher
Date: Dec 3, 2019，Views：1220
Original protocol used in research article：
NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress
eLife, Sep 30, 2019; DOI: 10.7554/eLife.49044
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