Protocols shared by authors via RaP service
Journal
CRISPR/Cas9 genome editing
Author: Morayma Temoche
Date: Nov 23, 2019，Views：1626
Original protocol used in research article：
Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes
eLife, Aug 22, 2019; DOI: 10.7554/eLife.47544
Fly stocks and genetics
Author: Helmut Krämer
Date: Nov 23, 2019，Views：1296
Original protocol used in research article：
Adaptation to constant light requires Fic-mediated AMPylation of BiP to protect against reversible photoreceptor degeneration
eLife, Jul 17, 2018; DOI: 10.7554/eLife.38752
SIM imaging
Author: Sue L Jaspersen
Date: Nov 21, 2019，Views：1375
Original protocol used in research article：
Structured illumination with particle averaging reveals novel roles for yeast centrosome components during duplication
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08586
Behavior
Author: Michael J Goard
Date: Nov 21, 2019，Views：1315
Original protocol used in research article：
Distinct roles of visual, parietal, and frontal motor cortices in memory-guided sensorimotor decisions
eLife, Aug 4, 2016; DOI: 10.7554/eLife.13764
ESC-derived motor neurons
Author: Esteban O. Mazzoni
Date: Nov 21, 2019，Views：1260
Original protocol used in research article：
Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons
eLife, Jun 3, 2019; DOI: 10.7554/eLife.44423
Influenza A genome sequencing
Author: Nacho Mena
Date: Nov 20, 2019，Views：1711
Original protocol used in research article：
Origins of the 2009 H1N1 influenza pandemic in swine in Mexico
eLife, Jun 28, 2016; DOI: 10.7554/eLife.16777
Desiccation survival
Author: Jennifer Sun
Date: Nov 18, 2019，Views：1102
Original protocol used in research article：
Humidity response depends on the small soluble protein Obp59a in Drosophila
eLife, Sep 19, 2018; DOI: 10.7554/eLife.39249
Observation and motility assay of zebrafish spermatozoa
Authors: Masahide Kikkawa and Hiroshi Yamaguchi
Date: Nov 18, 2019，Views：1294
Original protocol used in research article：
Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly
eLife, May 9, 2018; DOI: 10.7554/eLife.36979
Western blots
Author: Joel Hrit
Date: Nov 18, 2019，Views：1131
Original protocol used in research article：
OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function in development
eLife, Oct 16, 2018; DOI: 10.7554/eLife.34870
4TU metabolic labeling and RNA analysis
Author: Leon Chan
Date: Nov 18, 2019，Views：1550
Original protocol used in research article：
Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability
eLife, Sep 7, 2018; DOI: 10.7554/eLife.32536
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