Protocols shared by authors via RaP service
Journal
Electrophysiology and pharmacology for PHP
Authors: C Andrew Frank and Thomas D James
Date: May 26, 2020，Views：1118
Original protocol used in research article：
Maintenance of homeostatic plasticity at the Drosophila neuromuscular synapse requires continuous IP3-directed signaling
eLife, Jun 10, 2019; DOI: 10.7554/eLife.39643
DigiTAG – a RNA sequencing approach to analyze transcriptomes of rare cell populations in Drosophila melanogaster
Authors: Lisa Landskron, Francois Bonnay and Jürgen A Knoblich
Date: May 26, 2020，Views：1295
Original protocol used in research article：
The asymmetrically segregating lncRNA cherub is required for transforming stem cells into malignant cells
eLife, Mar 27, 2018; DOI: 10.7554/eLife.31347
VivosX for MCF10A Cells
Authors: Cheuk T Leung and Lisa M Kim
Date: May 26, 2020，Views：1091
Original protocol used in research article：
VivosX, a disulfide crosslinking method to capture site-specific, protein-protein interactions in yeast and human cells
eLife, Aug 9, 2018; DOI: 10.7554/eLife.36654
X34 staining
Author: Della C David
Date: May 26, 2020，Views：2460
Original protocol used in research article：
Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in Caenorhabditis elegans
eLife, May 3, 2019; DOI: 10.7554/eLife.43059
Protein network analysis
Author: Miguel A. Gonzalez-Lozano
Date: May 25, 2020，Views：974
Original protocol used in research article：
Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aax5783
Cell viability assays following treatment with aDT–eIF-3b
Author: Laura Smith
Date: May 24, 2020，Views：953
Original protocol used in research article：
Attenuated diphtheria toxin mediates siRNA delivery
Science Advances, Apr 30, 2020; DOI: 10.1126/sciadv.aaz4848
Tau expression and purification
Authors: Wenjuan Zhang, Michel Goedert and Sjors HW Scheres
Date: May 24, 2020，Views：6563
Original protocol used in research article：
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584
Immunohistochemistry (IHC) of DRG
Author: Diana M Bautista
Date: May 23, 2020，Views：3294
Original protocol used in research article：
The signaling lipid sphingosine 1-phosphate regulates mechanical pain
eLife, Mar 21, 2018; DOI: 10.7554/eLife.33285
hiPSC maintenance and differentiation
Authors: Swagata Ghatak, Dorit Trudler, Nima Dolatabadi and Stuart A Lipton
Date: May 22, 2020，Views：1338
Original protocol used in research article：
Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs isogenic controls
eLife, Nov 29, 2019; DOI: 10.7554/eLife.50333
Genus-level phylogenetic tree
Author: Ricardo A. Segovia
Date: May 22, 2020，Views：1097
Original protocol used in research article：
Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.