Protocols shared by authors via RaP service
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Pulldown assay
Author: Richard T Lee
Date: Jul 5, 2020，Views：1224
Original protocol used in research article：
Diabetes regulates fructose absorption through thioredoxin-interacting protein
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18313
Cell culture, nuclei isolation and GRO-seq library preparation
Authors: Iris Jonkers and John T Lis
Date: Jul 5, 2020，Views：1209
Original protocol used in research article：
Genome-wide dynamics of Pol II elongation and its interplay with promoter proximal pausing, chromatin, and exons
eLife, Apr 29, 2014; DOI: 10.7554/eLife.02407
In vivo antitumor therapy through AUNPs
Author: Duo Mao
Date: Jul 5, 2020，Views：1052
Original protocol used in research article：
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712
Human pluripotent stem cell culture and neural differentiation
Authors: Yan Liu and Fang Yuan
Date: Jul 4, 2020，Views：1145
Original protocol used in research article：
Induction of human somatostatin and parvalbumin neurons by expressing a single transcription factor LIM homeobox 6
eLife, Sep 25, 2018; DOI: 10.7554/eLife.37382
Preparation of cell samples with relatively uniform distribution of Alexa-647
Author: Yanxiang Ni
Date: Jul 3, 2020，Views：1045
Original protocol used in research article：
Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes
eLife, May 9, 2017; DOI: 10.7554/eLife.21660
Primary bone marrow stromal cultures
Author: Elie Traer
Date: Jul 2, 2020，Views：1018
Original protocol used in research article：
FGF2-FGFR1 signaling regulates release of Leukemia-Protective exosomes from bone marrow stromal cells
eLife, Feb 5, 2019; DOI: 10.7554/eLife.40033
In vitro B cell stimulation
Authors: Julie Zikherman and James L. Mueller
Date: Jul 2, 2020，Views：1920
Original protocol used in research article：
IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate
eLife, Mar 9, 2018; DOI: 10.7554/eLife.35074
Real-time qRT-PCR analysis
Authors: Ane Martin Anduaga and Sebastian Kadener
Date: Jul 2, 2020，Views：1362
Original protocol used in research article：
Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila
eLife, Nov 8, 2019; DOI: 10.7554/eLife.44642
RNA FISH
Author: Xiling Shen
Date: Jul 1, 2020，Views：1125
Original protocol used in research article：
A long non-coding RNA targets microRNA miR-34a to regulate colon cancer stem cell asymmetric division
eLife, Apr 14, 2016; DOI: 10.7554/eLife.14620
ECG recordings
Authors: Wenli Dai and Christopher R Weber
Date: Jul 1, 2020，Views：995
Original protocol used in research article：
A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice
eLife, Mar 21, 2019; DOI: 10.7554/eLife.41814
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