Protocols shared by authors via RaP service
Journal
Chromatin immunoprecipitation-deep sequencing (ChIP-seq) and ChIP-qPCR
Authors: Dong Fang and Zhiguo Zhang
Date: Jul 26, 2020，Views：3325
Original protocol used in research article：
H3.3K27M mutant proteins reprogram epigenome by sequestering the PRC2 complex to poised enhancers
eLife, Jun 22, 2018; DOI: 10.7554/eLife.36696
Human embryonic lung organoid culture including recovering organoids from matrigel for immunostaining or RNA extraction
Authors: Emma L Rawlins and Dawei Sun
Date: Jul 23, 2020，Views：1445
Original protocol used in research article：
Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids
eLife, Jun 30, 2017; DOI: 10.7554/eLife.26575
cLTP and cLTD protocols
Author: Okunola Jeyifous
Date: Jul 22, 2020，Views：1656
Original protocol used in research article：
Synaptic activity regulates AMPA receptor trafficking through different recycling pathways
eLife, May 13, 2015; DOI: 10.7554/eLife.06878
Block-bootstrap procedure
Author: Fanny Pouyet
Date: Jul 21, 2020，Views：1316
Original protocol used in research article：
Background selection and biased gene conversion affect more than 95% of the human genome and bias demographic inferences
eLife, Aug 23, 2018; DOI: 10.7554/eLife.36317
In vitro effects of YAP1-targeting drugs
Authors: Tomohiko Maehama, Akira Suzuki and Hirofumi Omori
Date: Jul 21, 2020，Views：914
Original protocol used in research article：
YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma
Science Advances, Mar 17, 2020; DOI: 10.1126/sciadv.aay3324
Overexpression and purification of the recombinant β-actin inclusion-body protein
Author: Jakub Drozak
Date: Jul 21, 2020，Views：1081
Original protocol used in research article：
Structural insights into SETD3-mediated histidine methylation on β-actin
eLife, Feb 20, 2019; DOI: 10.7554/eLife.43676
Calculation of residue-residue contact score (RRCS)
Authors: Qingtong Zhou, Ming-Wei Wang and Suwen Zhao
Date: Jul 21, 2020，Views：1554
Original protocol used in research article：
Common activation mechanism of class A GPCRs
eLife, Dec 19, 2019; DOI: 10.7554/eLife.50279
Expression and purification of SpyCas9
Authors: Xinyu Ling and Tao Liu
Date: Jul 20, 2020，Views：1065
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Phagocytosis assay
Authors: Alexandre B Leitão and Élio Sucena
Date: Jul 20, 2020，Views：987
Original protocol used in research article：
Drosophila sessile hemocyte clusters are true hematopoietic tissues that regulate larval blood cell differentiation
eLife, Feb 4, 2015; DOI: 10.7554/eLife.06166
Barcode association of library variants
Authors: Julia M Flynn and Daniel NA Bolon
Date: Jul 20, 2020，Views：1093
Original protocol used in research article：
Comprehensive fitness maps of Hsp90 show widespread environmental dependence
eLife, Mar 4, 2020; DOI: 10.7554/eLife.53810
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