Protocols shared by authors via RaP service
Journal
Molecular dynamics simulations
Author: Gregory R Bowman
Date: Aug 12, 2020，Views：945
Original protocol used in research article：
Conformational distributions of isolated myosin motor domains encode their mechanochemical properties
eLife, May 29, 2020; DOI: 10.7554/eLife.55132
Quantitative Real-Time PCR
Author: Youngah Jo
Date: Aug 12, 2020，Views：979
Original protocol used in research article：
Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice
eLife, Feb 20, 2019; DOI: 10.7554/eLife.44396
DNA prep for SNP TaqMan assay
Authors: Huan Liu and Robert A Cornell
Date: Aug 12, 2020，Views：1018
Original protocol used in research article：
Analysis of zebrafish periderm enhancers facilitates identification of a regulatory variant near human KRT8/18
eLife, Feb 7, 2020; DOI: 10.7554/eLife.51325
Cytokine quantification
Authors: Claudio R. F. Marinho and Aramys Silva Reis
Date: Aug 10, 2020，Views：877
Original protocol used in research article：
Inflammasome activation and IL-1 signaling during placental malaria induce poor pregnancy outcomes
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aax6346
Cerebral and ChP organoid culture conditions
Author: Laura Pellegrini
Date: Aug 10, 2020，Views：1322
Original protocol used in research article：
Human CNS barrier-forming organoids with cerebrospinal fluid production
Science, Jul 9, 2020; DOI: 10.1126/science.aaz5626
Antibodies and flow cytometry
Author: Z. Zhang
Date: Aug 10, 2020，Views：931
Original protocol used in research article：
Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aaz0374
The novel trial set
Author: Sebastian Gluth
Date: Aug 7, 2020，Views：966
Original protocol used in research article：
Value-based attentional capture affects multi-alternative decision making
eLife, Nov 5, 2018; DOI: 10.7554/eLife.39659
Immunofluorescence staining protocol to detect ssDNA (CldU) and active replication (EdU)
Authors: Sumeet Nayak and Sharon B Cantor
Date: Aug 5, 2020，Views：1500
Original protocol used in research article：
Inhibition of the translesion synthesis polymerase REV1 exploits replication gaps as a cancer vulnerability
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz7808
Zebrafish strains, husbandry, embryo treatments, and morpholinos
Author: Julien Vermot
Date: Aug 5, 2020，Views：989
Original protocol used in research article：
Mechanically activated piezo channels modulate outflow tract valve development through the Yap1 and Klf2-Notch signaling axis
eLife, Sep 16, 2019; DOI: 10.7554/eLife.44706
Lysosomal exocytosis assay (Hexosaminidase)
Authors: Elisabeth S Butz and Christian Grimm
Date: Aug 5, 2020，Views：3573
Original protocol used in research article：
Selective agonist of TRPML2 reveals direct role in chemokine release from innate immune cells
eLife, Nov 27, 2018; DOI: 10.7554/eLife.39720
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