Protocols shared by authors via RaP service
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Extraction and purification of the photosynthetic ACIII from R. castenholzii
Authors: Yueyong Xin and Xiaoling Xu
Date: Aug 18, 2020，Views：871
Original protocol used in research article：
Cryo-EM structures of the air-oxidized and dithionite-reduced photosynthetic alternative complex III from Roseiflexus castenholzii
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aba2739
Acute extracellular electrophysiology in mouse primary visual cortex
Authors: Arbora Resulaj, Sarah Ruediger, Shawn R Olsen and Massimo Scanziani
Date: Aug 18, 2020，Views：1176
Original protocol used in research article：
First spikes in visual cortex enable perceptual discrimination
eLife, Apr 16, 2018; DOI: 10.7554/eLife.34044
Rubicon ThruPlex DNA-Seq library preparation protocol
Author: Laura A Lavery
Date: Aug 18, 2020，Views：1036
Original protocol used in research article：
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Western blotting
Author: Daniel T Hughes
Date: Aug 18, 2020，Views：921
Original protocol used in research article：
Targeting the kinase insert loop of PERK selectively modulates PERK signaling without systemic toxicity in mice
Science Signaling, Aug 10, 2020; DOI: 10.1126/scisignal.abb4749
Cell culture and treatment
Authors: Hyungsoo Kim and Ze’ev A. Ronai
Date: Aug 17, 2020，Views：988
Original protocol used in research article：
PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aaz5683
Preparation of SiUNPs, AUNPs, and pAUNPs
Author: Duo Mao
Date: Aug 17, 2020，Views：905
Original protocol used in research article：
AIEgen-coupled upconversion nanoparticles eradicate solid tumors through dual-mode ROS activation
Science Advances, Jun 25, 2020; DOI: 10.1126/sciadv.abb2712
In situ hybridization and antibody labeling
Author: Catherine M Drerup
Date: Aug 14, 2020，Views：960
Original protocol used in research article：
Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons
eLife, Apr 17, 2017; DOI: 10.7554/eLife.22234
Construction and small-scale production of hIgG1Fc-VHH chimeric antibodies
Author: Dubravka Drabek
Date: Aug 12, 2020，Views：1057
Original protocol used in research article：
Multimeric single-domain antibody complexes protect against bunyavirus infections
eLife, Apr 21, 2020; DOI: 10.7554/eLife.52716
Synthesis of SCLMs
Author: Lingzhi Zhao
Date: Aug 12, 2020，Views：843
Original protocol used in research article：
MTH1 inhibitor amplifies the lethality of reactive oxygen species to tumor in photodynamic therapy
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aaz0575
Preparation and characterization of nanomedicine-shelled microbubbles
Authors: Yu Gao and Si yu Wang
Date: Aug 12, 2020，Views：876
Original protocol used in research article：
Accelerating thrombolysis using a precision and clot-penetrating drug delivery strategy by nanoparticle-shelled microbubbles
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aaz8204
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