Protocols shared by authors via RaP service
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Measurement of transcriptional drift and drift-variance
Author: Michael Petrascheck
Date: Aug 27, 2020，Views：1031
Original protocol used in research article：
Suppression of transcriptional drift extends C. elegans lifespan by postponing the onset of mortality
eLife, Dec 1, 2015; DOI: 10.7554/eLife.08833
Recombinant oligonucleosomes
Authors: Fangfang Jiao and Rui Guo
Date: Aug 27, 2020，Views：821
Original protocol used in research article：
RACK7 recognizes H3.3G34R mutation to suppress expression of MHC class II complex components and their delivery pathway in pediatric glioblastoma
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba2113
PCR-RFLP of G1 and G2 mutations in the human Apolipoprotein-L1 (APOL1) gene
Author: Anneli Cooper
Date: Aug 25, 2020，Views：1406
Original protocol used in research article：
APOL1 renal risk variants have contrasting resistance and susceptibility associations with African trypanosomiasis
eLife, May 24, 2017; DOI: 10.7554/eLife.25461
ML algorithms and performance measures
Author: Juliana Bernardes
Date: Aug 25, 2020，Views：869
Original protocol used in research article：
Structure-specific DNA recombination sites: Design validation and machine learning–based refinement
Science Advances, Jul 23, 2020; DOI: 10.1126/sciadv.aay2922
Immunostaining
Author: Junhua Yang
Date: Aug 24, 2020，Views：954
Original protocol used in research article：
Astrocytes contribute to synapse elimination via type 2 inositol 1,4,5-trisphosphate receptor-dependent release of ATP
eLife, Apr 12, 2016; DOI: 10.7554/eLife.15043
Encapsulated Rapamycin Feeding Protocol
Authors: Matt Kaeberlein and Jonathan An
Date: Aug 24, 2020，Views：1535
Original protocol used in research article：
Rapamycin rejuvenates oral health in aging mice
eLife, Apr 28, 2020; DOI: 10.7554/eLife.54318
Signal pathway investigation
Authors: Funeng Xu and Shaobing Zhou
Date: Aug 23, 2020，Views：827
Original protocol used in research article：
Development of a pH-responsive polymersome inducing endoplasmic reticulum stress and autophagy blockade
Science Advances, Jul 30, 2020; DOI: 10.1126/sciadv.abb8725
Measurement of mtDNA copy number and quantification of heteroplasmy
Author: Zong-Heng Wang
Date: Aug 21, 2020，Views：1247
Original protocol used in research article：
Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila
eLife, Oct 15, 2019; DOI: 10.7554/eLife.49309
GST pull-down assay
Author: Shen Wang
Date: Aug 21, 2020，Views：1229
Original protocol used in research article：
Synaptotagmin-1 C2B domain interacts simultaneously with SNAREs and membranes to promote membrane fusion
eLife, Apr 15, 2016; DOI: 10.7554/eLife.14211
Western blotting and immunoprecipitation
Authors: Hozaifa Metwallly and Tadamitsu Kishimoto
Date: Aug 20, 2020，Views：1691
Original protocol used in research article：
Noncanonical STAT1 phosphorylation expands its transcriptional activity into promoting LPS-induced IL-6 and IL-12p40 production
Science Signaling, Mar 23, 2020; DOI: 10.1126/scisignal.aay0574
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