Protocols shared by authors via RaP service
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Preparation of brain samples for immunofluorescence, immunoblot, and nucleic acid analysis
Author: Bradlee L. Heckmann
Date: Sep 8, 2020，Views：867
Original protocol used in research article：
Noncanonical function of an autophagy protein prevents spontaneous Alzheimer’s disease
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.abb9036
Thermostability shift assay and library screening
Author: Edmund R.S. Kunji
Date: Sep 7, 2020，Views：962
Original protocol used in research article：
Screening of candidate substrates and coupling ions of transporters by thermostability shift assays
eLife, Oct 15, 2018; DOI: 10.7554/eLife.38821
In vitro 2d matrigel migration and protrusion assay
Authors: Monte M Winslow, Julien Sage and Dian Yang
Date: Sep 6, 2020，Views：1058
Original protocol used in research article：
Axon-like protrusions promote small cell lung cancer migration and metastasis
eLife, Dec 13, 2019; DOI: 10.7554/eLife.50616
Anti-aggregation assays
Author: Tomohide Saio
Date: Sep 4, 2020，Views：988
Original protocol used in research article：
Oligomerization of a molecular chaperone modulates its activity
eLife, May 1, 2018; DOI: 10.7554/eLife.35731
WorMotel preparation
Author: Christopher Fang-Yen
Date: Sep 4, 2020，Views：1350
Original protocol used in research article：
Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging
eLife, May 31, 2017; DOI: 10.7554/eLife.26652
Yeast strains and growth media
Authors: Abbas Jariani, Lieselotte Vermeersch and Kevin J Verstrepen
Date: Sep 4, 2020，Views：1140
Original protocol used in research article：
A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast
eLife, May 18, 2020; DOI: 10.7554/eLife.55320
scRNA-seq using the 10x Genomics platform
Authors: Abbas Jariani, Lieselotte Vermeersch and Kevin J Verstrepen
Date: Sep 4, 2020，Views：1591
Original protocol used in research article：
A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast
eLife, May 18, 2020; DOI: 10.7554/eLife.55320
Zebrafish genotyping
Authors: Kelly R Monk and Xianhua Piao
Date: Sep 3, 2020，Views：917
Original protocol used in research article：
Microglial transglutaminase-2 drives myelination and myelin repair via GPR56/ADGRG1 in oligodendrocyte precursor cells
eLife, May 29, 2018; DOI: 10.7554/eLife.33385
Neonatal transverse sinus injections for whole-brain gene expression
Authors: Ali S Hamodi and Michael Crair
Date: Sep 2, 2020，Views：3473
Original protocol used in research article：
Transverse sinus injections drive robust whole-brain expression of transgenes
eLife, May 18, 2020; DOI: 10.7554/eLife.53639
Pair-wise phase consistency for LFP-LFP phase synchrony
Author: Lu Zhang
Date: Sep 2, 2020，Views：1964
Original protocol used in research article：
Sub-second dynamics of theta-gamma coupling in hippocampal CA1
eLife, Jul 29, 2019; DOI: 10.7554/eLife.44320
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