Protocols shared by authors via RaP service
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Quantification of tracheal density in IFMs
Author: Stefan Luschnig
Date: Dec 4, 2019，Views：1181
Original protocol used in research article：
Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857
Growth chamber experiments
Author: Steven Penfield
Date: Dec 4, 2019，Views：1184
Original protocol used in research article：
Flowering time and seed dormancy control use external coincidence to generate life history strategy
eLife, Mar 31, 2015; DOI: 10.7554/eLife.05557
Isolation of skeletal muscle-derived mononuclear cells
Author: So-ichiro Fukada
Date: Dec 3, 2019，Views：1077
Original protocol used in research article：
Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle
eLife, Sep 23, 2019; DOI: 10.7554/eLife.48284
Purification of mammalian dynein-dynactin
Author: Ahmet Yildiz
Date: Dec 3, 2019，Views：1159
Original protocol used in research article：
Kinesin and dynein use distinct mechanisms to bypass obstacles
eLife, Sep 9, 2019; DOI: 10.7554/eLife.48629
In vitro differentiation of mESCs in embryoid bodies
Author: Lars Schomacher
Date: Dec 3, 2019，Views：1218
Original protocol used in research article：
NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress
eLife, Sep 30, 2019; DOI: 10.7554/eLife.49044
Platelet isolation and quantification
Author: Mercedes Costell
Date: Dec 1, 2019，Views：1144
Original protocol used in research article：
The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes
eLife, Jan 16, 2017; DOI: 10.7554/eLife.22264
Preparation of mini-Gs heterotrimer
Author: Christopher Gordon Tate
Date: Dec 1, 2019，Views：1725
Original protocol used in research article：
Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein
eLife, May 4, 2018; DOI: 10.7554/eLife.35946
High-resolution and deep phylogenetic reconstruction of ancestral states from large transcriptomic data sets
Author: Sumanth Mutte
Date: Nov 27, 2019，Views：1338
Original protocol used in research article：
Origin and evolution of the nuclear auxin response system
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33399
GFP-based Luria-Delbrück fluctuation test
Author: Adam S Lauring
Date: Nov 27, 2019，Views：1509
Original protocol used in research article：
A novel twelve class fluctuation test reveals higher than expected mutation rates for influenza A viruses
eLife, Jun 9, 2017; DOI: 10.7554/eLife.26437
Plasmids generation
Author: Harihar Basnet
Date: Nov 24, 2019，Views：1140
Original protocol used in research article：
Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43627
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