摘要:用稳定同位素标记的初始物处理活的生物组织,观测不同时间点下游代谢物的被标记比例可以反映该组织的代谢速率。现以全碳标记的葡萄糖处理果蝇幼虫脂肪组织为例,描述如何检测果蝇组织糖类代谢速率 (Ding等, 2018)。
关键词: 同位素, 葡萄糖, 代谢速率, 果蝇, 脂肪组织
材料与试剂
仪器设备
实验步骤
溶液配方
参考文献
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