摘要:采用商业化试剂盒提取草莓组织中的总RNA,以其中的mRNA为模板,采用Oligo dT在逆转录酶的作用下反转为cDNA,再以cDNA为模板进行PCR扩增,从而检测目的基因的表达。RT-PCR可对目的基因的表达丰度及基因间表达的差异进行检测,易于操作,周期短。
关键词: 草莓, 基因表达, PCR
材料与试剂
仪器设备
实验步骤
注意事项
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