This is a correction notice. See the corrected protocol.

Correction Notice: Three-dimensional Models of the Nasopharynx for the Study of Epstein-Barr Virus Infection

Published: Jan 20, 2026 DOI: 10.21769/BioProtoc.5603 Views: 11

Reviewed by: Anonymous reviewer(s)

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After official publication of our protocol in Bio-protocol (https://bio-protocol.org/e4365), the authors would like to update the Acknowledgement section as follows:

Acknowledgements
We thank Mary Ferguson and Ian Hayman for their help with optimizing the organotypic rafts. K.S. received funding from the Hillman Foundation. M. M. received funding from the Cystic Fibrosis Foundation Research Development Program (to the University of Pittsburgh) through the Cystic Fibrosis Research Center Cell Core. This work was supported in part by the University of Pittsburgh School of Medicine Dean’s Faculty Advancement Award, the National Institutes of Health R01 AI168022 (to KHY Shair), and used the Hillman Tissue and Research Pathology Services shared resource that is supported in part by the National Institutes of Health award P30CA047904. This protocol is based on the manuscript “A primary nasopharyngeal three-dimensional air-liquid interface cell culture model of the pseudostratified epithelium reveals differential donor- and cell type-specific susceptibility to Epstein-Barr virus infection” (Ziegler et al., 2021). The protocol for EBV infection in organotypic rafts was adapted from the manuscript “Generation and Infection of Organotypic Cultures with Epstein-Barr Virus” (Temple et al., 2017).

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