An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos
[Abstract] In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
Reprogram Murine Epiblast Stem Cells by Epigenetic Inhibitors
Authors: Hui Zhang and
Yali Dou,
date: 03/05/2017,
view: 3929,
Q&A: 0
[Abstract] Pluripotent stem cells in the naïve state are highly useful in regenerative medicine and tissue engineering. A robust reprogramming of the primed murine Epiblast Stem Cells (EpiSCs) to naïve pluripotency is feasible via chemical-only approach. This protocol described a method to reprogram murine EpiSCs by MM-401 treatment, which blocks histone ...
Differentiation of Human Embryonic Stem Cells into Cone Photoreceptors
[Abstract] Photoreceptors are specialized retinal neurons able to respond to light in order to generate visual information. Among photoreceptors, cones are involved in colors discrimination and high-resolution central vision and are selectively depleted in macular degenerations and cone dystrophies. A possible therapeutic solution for these disorders is to ...