Andreas Gewies Helmholtz Zentrum Munich
1 protocol

Daniel Krappmann Molecular Targets and Therapies Center (MTTC) Munich
2 protocols

Thomas J. O'Neill
  • Post-Doc, Helmholtz Zentrum Munich
  • 1 Author merit


PhD, Ludwig-Maximilians-Universität München, 2021

Lab information

Daniel Krappmann Lab

1 Protocol published
Optimized CRISPR-Cas9-based Strategy for Complex Gene Targeting in Murine Embryonic Stem Cells for Germline Transmission
Authors:  Thomas J. O'Neill, Daniel Krappmann and Andreas Gewies, date: 05/20/2022, view: 1222, Q&A: 0

Although CRISPR-Cas9 genome editing can be performed directly in single-cell mouse zygotes, the targeting efficiency for more complex modifications such as the insertion of two loxP sites, multiple mutations in cis, or the precise insertion or

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