Melissa L. Knothe Tate
  • Professor, Paul Trainor Chair of Biomedical Engineering, Graduate School of Biomedical Engineering, University of New South Wales, Australia
Research fields
  • Cell biology
Personal information


Ph.D., ETH Zurich, 1998

Lab information

MechBio Team

Research focus

Engineering at the Interface of Mechanics & Biology: from Cellular Bioengineering to Development of Disruptive Tech & Next Generation Implants

Multiscale orthopaedic mechanobiology in healing, health & disease states

Pairing cross scale and multimodal imaging with computational tech as an enabling platform

Multi-scale computational and experimental mechanobiology: applying computational and experimental methods to uncover and facilitate the biophysical mechanisms underlying processes of development, growth, adaptation and repair of biological systems, at the cellular, tissue and organ level

Stem cell mechanics & mechanobiology: harnessing this capacity for regenerative medicine and therapeutic devices

Engineering & development of mechano-active, bio-inspired, and/or novel materials (e.g. tissues) & implants cum delivery devices

Ngo L.*, Nathanson, A.*, Garbowski T., Knothe U., Zeidler D., Tate M.L.K. (2019) Sample preparation protocol enabling nano-to-mesoscopic mapping of cellular connectomes and their habitats in human tissues and organs, Bio-protocols, in press.

Hageman D.J., Wu S., Latimer D., Virdi K., Wang C.H., and Knothe Tate M.L. (2019) Comprehensive pressure profiling to develop next-generation compression treatment for lymphedema: testing efficacy of high resolution sensors, Sensors and Actuators,

Sidler HJ, Duvenage J, Anderson EJ, Ng J, Hageman DJ, Knothe Tate ML (2018) Prospective design, rapid prototyping and testing of smart dressings, drug delivery patches, and replacement body parts using Microscopy Aided Design And ManufacturE (MADAME), Frontiers in Medicine | Translational Medicine,

Ngo L., Knothe L., Tate M.L.K., (2018) Tissues of the Knee Joint Effectively Separate Mixed Sized Molecules Delivered Simultaneously via the Heart, Nature Scientific Reports, 8, 10254.

Hageman D., Wu S., Kilbreath S., Rockson S.G., Wang C., Knothe Tate M.L. (2018) Towards a Cure and Ultimately Prevention: State-of-the-Art & Next Generation Biotechnologies for Edema Mitigating Compression Therapy, Trends in Biotechnology, 36(5): 537-548.

Knothe Tate M.L. (2017) Navigation of bee brains to human hips - microscopy and the modern Magellans. in: A new age in scanning electron microscopy: Applications in the life sciences (Science/ AAAS, Washington, DC, 2017), p. 19-23.

Ng J., Kilbreath S., Kersch M., Knothe Tate M.L. (2017) Establishing the Basis for Mechanobiology-Based Physical Therapy Protocols to Potentiate Cellular Healing and Tissue Regeneration, Frontiers in Physiology - Exercise Physiology,

Ng J.L., Collins C.E., Knothe Tate M.L. (2017) Engineering mechanical gradients in next generation biomaterials – lessons learned from medical textile design, Acta Biomaterialia, 56:14 – 24.

Yu N., O'Brien C.A., Slapetova I., Whan R., Knothe Tate M.L. (2017) Live tissue imaging to elucidate mechanical modulation of stem cell niche quiescence. Stem Cells Translational Medicine, 6(1): 282-292, doi:10.5966/sctm.2015-0306.

Ng J., Knothe L., Whan R., Knothe U., Knothe Tate M.L. (2017) Scale-up of Nature's Tissue Weaving Algorithms to Engineer Advanced Functional Materials, Nature Sci Reports, 7: 40396, doi: 10.1038/srep40396.

Hageman D., Pereira A., Zeidler D., Knothe U., Gardner L., Knothe Tate M.L. (2016) Cellular Epidemiology of Human Disease using Biogeographic and Google Maps Approaches - Towards Definition of Cell Network Indices for Rapid Diagnostics. Annals of Biomedical Engineering, 44:3719, doi:

Pereira A., Hageman D., Knothe U., Zeidler D., Knothe Tate M.L. (2016) Creating high-resolution multiscale maps of human tissue using multi-beam SEM. PLoS Comp Biol 12(11): e1005217.

Knothe Tate M.L., Yu N.Y.C., Jalilian I., Pereira A., Knothe U.R. (2016) Periosteum Mechanobiology and Mechanistic Insights for Regenerative Medicine. Nature Publishing Group BoneKey Reports 5: 857, doi: 10.1038/bonekey.2016.70.

Knothe Tate M.L., Fath T. (2016) The only constant is change: next generation medical devices for physical and mental health, Advanced Healthcare Materials, 5(15): 1840-3. doi: 10.1002/adhm.201600437.

Moore S.R., Heu C., Yu N., Whan R., Knothe U., Milz S., Knothe Tate M.L. (2016) Translating Periosteum's Regenerative Power: Insights from Quantitative Analysis of Tissue Genesis with a Periosteum Substitute Implant, Stem Cells Translational Medicine, 5:1739-1749.

Knothe Tate M.L., Gunning P., Sansalone V. (2016) Emergence of form from function: role of stem cell mechanoadaptation in sealing of cell fate. BioArchitecture 6(5): 85-103.

Knothe Tate M.L., Zeidler D., Pereira A., Hageman D., Garbowski T., Mishra S., Gardner L., Knothe U. (2016) Organ-to-Cell-Scale Health Assessment using Google Maps API and Navigation Approaches, Advanced Healthcare Materials 5(13): 1581-87.

Knothe Tate M.L., Detamore M., Capadona J., Wooley A., Knothe U. (2016) Engineering and Commercialization of Human-Device Interfaces, from Bone to Brain, Biomaterials, 95: 35-46.

Schemesh J., Jalilian I.H.P., Shi A., Knothe Tate M.L., Yeoh G.H., Ebrahimi Warkiani M. (2015) Flow induced stress on adherent cells in microfluidic devices, Lab on a Chip 15(21):4114-27.

Eberle A.-L., Mikula S., Schalek R., Lichtman J., Knothe Tate M.L., Zeidler D. (2015) High-resolution, high-throughput imaging with a multiple beam scanning electron microscope. J Microscopy 259(2):114-20,

Moore S., Saidel G., Knothe U., Knothe Tate M.L. (2014) Mechanistic, Mathematical Model to Predict the Dynamics of Tissue Genesis in Bone Defects via Mechanical Feedback and Mediation of Biochemical Factors, PLoS Comp Biol 10(6):e1003604.

Chang H., Docheva D., Knothe U.R., Knothe Tate M.L. (2014) Arthritic periosteal tissue from joint replacement surgery as an autologous source of stem cells, Stem Cells Translational Medicine 3(3):308-17.

Moore S.R, Milz S., Knothe Tate M.L. (2014) Periosteal thickness and cellularity in middiaphyseal cross sections from human femora and tibiae of aged donors, Journal of Anatomy 224(2): 124-9.

Moore S., Milz S., Knothe Tate M.L. (2014) The linea aspera: a virtual case study testing emergence of form and function, Anatomical Record, 297(2): 273-80.

Freutel M, Seitz AM, Galbusera F, Bornstedt A, Rasche V, Knothe Tate ML, Ignatius A., Dörselen L. (2014) Medial meniscal displacement and strain in three dimensions under compressive loads: MR assessment, Journal of Magnetic Resonance Imaging, doi: 10.1002/jmri.24461.

Knothe Tate M.L., O'Leary J., McNamara E., Cai L., Knothe U. (2013) Lithotripsy stimulates new bone formation and mitigates loss of bone due to disuse in aged rats, Tech Health Care 21(6): 587-97.

Song M.J., Dean D., Knothe Tate M.L. (2013) Mechanical Modulation of Nascent Cell Lineage Commitment in Tissue Engineering Scaffolds, Biomaterials, 34(23): 5766-75.

Evans S., Chang H., Knothe Tate M.L. (2013) Elucidating multiscale periosteal mechanobiology: A key to unlocking the regenerative capacity of the periosteum? Tissue Engineering Part B, 19(2): 147-59.

Evans S., Parent J., Lasko C., Zheng X., Knothe U., Lemaire T., Knothe Tate M.L. (2013) Periosteum, Bone's "Smart" Bounding Membrane, Exhibits Direction Dependent Permeability, Journal of Bone and Mineral Research 28(3): 608-17.

Evans S.F., Docheva D., Bernecker A., Colnot C., Richter R., Knothe Tate M.L. (2013) Solid-Supported Lipid Bilayers as a Novel Platform to Engineer Emergence of Stem Cell Fate and Tissue Architecture using Periosteum Derived Progenitor Cells, Biomaterials, 34(8):1878-1887.

Chang H., Knothe Tate M.L. (2012) Culturing Stem Cells to Achieve Physiological Contexts and to Maximize Clinical Relevance, Case Orthopaedic Journal 9(1): 40-43.

Song M.J., Brady-Kalnay S., Phillips-Mason P., Dean D., Knothe Tate M.L. (2012) Mapping the mechanome of live stem cells: using a novel method to measure local strain fields in situ at the fluid-cell interface, PLoS one, 7(9): e43601.

Knothe Tate M.L., Tami A., Netrebko P., Milz S., Docheva D. (2012) Multiscale computational and experimental approaches to elucidate musculoskeletal and ligament mechanobiology using the ulna-radius-interosseous membrane construct as a model system, Tech Health Care, 20(5):363-78.

Colnot C., Zhang X., Knothe Tate M.L. (2012) Current Insights on the Regenerative Potential of the Periosteum: Molecular, Cellular and Endogenous Engineering Approaches, Journal of Orthopaedic Research, 30(12):1869-78.

Chang H., Knothe Tate M.L. (2012) The periosteum: tapping into a reservoir of clinically useful progenitor cells, Stem Cells Translational Medicine, 1(6): 480-491.

Zimmerman J., Knothe Tate M.L. (2011) Structure - function relationships in the stem cell's mechanical world A: Seeding protocols as a means to control shape and fate in live cells, Molecular and Cellular Biomechanics, 8(4): 275-296.

Chang H., Knothe Tate M.L. (2011) Structure - function relationships in the stem cell's mechanical world B: Emergent anisotropy of the cytoskeleton correlates to volume and shape changing stress exposure, Molecular and Cellular Biomechanics, 8(4): 297-318.

Knothe Tate M.L., Moore S., Chang H., Knothe U. (2011) Surgical Membranes as Directional Delivery Devices to Generate Tissue in Critical Sized Defects, PLoS one, 6(12): e28702. PMID: 22174873. 525 views in first two weeks after publication.

McBride S.H., Evans S., Knothe Tate M.L. (2011) Anisotropic mechanical properties of ovine femoral periosteum and the effects of cryopreservation. Journal of Biomechanics 44(10): 1954-9.

Knothe Tate M.L., Dolejs S., McBride S., Miller R.M., Knothe U.R. (2011) Multiscale Mechanobiology of De Novo Bone Generation as well as Remodeling & Adaptation of Autograft - An Integrative Review, Journal of the Mechanical Behavior of Biomedical Materials 4(6): 829-40.

McBride S.H., Dolejs S., Miller R.M., Knothe U., Knothe Tate M.L. (2011) Major and Minor Centroidal Axes Serve as Absolute Reference Points to Test Mechanobiological Hypotheses Using Histomorphometry, Journal of Biomechanics 44(6):1205-1208.

McBride S.H., Dolejs S., Brianza S., Knothe U., Knothe Tate M.L. (2011) Net Change in Periosteal Strain Correlates to Rapid De Novo Bone Generation in Critical Sized Defects, Annals of Biomedical Engineering 39(5): 1570-1581.

Knothe Tate M.L. (2011) Smart body armor inspired by flow in bone. Smart Structures & Systems, 7(3): 223-228.

Knothe Tate M.L. (2011) Top down and bottom up engineering of bone. Journal of Biomechanics, 44(2): 304-12.

Song M.J., Dean D., Knothe Tate M.L. (2010) In situ spatiotemporal mapping of flow fields around seeded stem cells at the subcellular length scale, PLoS one, 5(9), pii: e12796, 563 views in the first week after publication.

Knothe U. Dolejs S., Miller R.M., Knothe Tate M.L. (2010) Effects of mechanical loading patterns, bone graft, and proximity to periosteum on bone defect healing, Journal of Biomechanics, 43(14): 2728-37.

Knothe Tate M.L., Dolejs S., Miller R.M., Knothe U. (2010) Role of mechanical loading in healing of massive bone autografts, Journal of Orthopaedic Research 28(12): 1657-84.

Knothe Tate M.L., Falls T., S. Mishra, Atit R. (2010) Engineering an ecosystem: taking cues from nature’s paradigm to build tissue in the lab and the body, Fields Institute for Mathematics in Biology monograph series on New Perspectives in Mathematical Biology, 57: 113-134.

Knothe Tate M.L., Steck R., Anderson E.J. (2009) Bone as an inspiration for a novel class of biomaterials, Biomaterials, 30: 133-140.

Knothe Tate M.L., Falls T., McBride S.H., Atit R., Knothe U.R. (2008) Mechanical modulation of osteochondroprogenitor cell fate, The International Journal of Biochemistry and Cell Biology 40(12): 2720-2738.

McBride S.H., Knothe Tate M.L. (2008), Modulation of stem cell shape and fate, A: Role of density and seeding protocol on nucleus shape and gene expression. Tissue Engineering, 14(9): 1561-1572.

McBride S.H., Falls T., Knothe Tate M.L. (2008), Modulation of stem cell shape and fate, B: Mechanical modulation of cell shape and gene expression. Tissue Engineering, 14(9): 1573-1580.

Anderson E.J., Knothe Tate M.L. (2008) Idealization of pericellular fluid space geometry and dimension results in a profound underprediction of nano-microscale stresses imparted by fluid drag on osteocytes, Journal of Biomechanics, 41(8): 1736-1746.

Anderson E.J., Kreuzer S.M., Small O., Knothe Tate M.L. (2008) Pairing computational and scaled physical models to determine permeability as a measure of cellular communication in micro- and nano-scale pericellular spaces. Microfluidics and Nanofluidics, 4:193-204.

Mishra S.K., Knothe Tate M. (2008) Comparative study of bone micro-architecture of some mammalian bones. The Paleobotanist 57 (1-2), 299-302.

Anderson E.J., Knothe Tate M.L. (2007) Open access to novel flow chamber technology for in vitro cell mechanotransduction, toxicity and pharmacokinetic studies, BioMedical Engineering OnLine, 6:46.

Anderson E.J., Knothe Tate M.L. (2007) Design of tissue engineering scaffolds as delivery devices for mechanical and mechanically modulated signals. Tissue Engineering, 13(10): 2525-2538.

Knothe Tate M.L., Ritzman T., Schneider E., Knothe U. (2007) Testing of a new one-stage bone-transport surgical procedure exploiting the periosteum and bone transport for repair of long bone defects, Journal of Bone and Joint Surgery American, 89(2): 307-316.

Anderson E.J., Falls T.D., Sorkin A., Knothe Tate M.L. (2006) The imperative for controlled mechanical stresses in unraveling cellular mechanisms of mechanotransduction. BioMedical Engineering OnLine, 5:27.

Steck R., Knothe Tate M.L. (2005) In silico stochastic network models that emulate the molecular sieving characteristics of bone, Annals of Biomedical Engineering, 33(1): 87-94.

Anderson E.J., Kaliyamoorthy S., Alexander J.I.D., Knothe Tate M.L. (2005) Nano-Microscale Models of Periosteocytic Flow Show Differences in Stresses Imparted to Cell Body and Processes, Annals of Biomedical Engineering, 33(1): 52-62.

Sorkin A., Dee KC, Knothe Tate M.L. (2004) „Culture Shock“ from the Bone Cell’s Perspective: Emulating Physiologic Conditions for Mechanobiological Investigation, Am J Physiol Cell Physiol 287(6):C1527-36.

Knothe Tate M.L., Adamson J.R., Tami A.E., Bauer T.W. (2004) The Osteocyte, The International Journal of Biochemistry and Cell Biology 36(1): 1-8.

Tami A., Nasser P., Schaffler M.B., Knothe Tate M.L. (2003) Nonnvasive fatigue fracture model of the rat ulna, Journal Orthopaedic Research, 21(6): 1018-1024.

Tami A., Schaffler M.B., Knothe Tate M.L. (2003) Probing the tissue to subcellular level structure underlying bone’s molecular sieving function, Biorheology, 40(6): 577-590.

Knothe Tate M.L. (2003) “Whither flows the fluid in bone?”: An Osteocyte’s Perspective, Journal of Biomechanics, 36(10), 1409-1424.

Mishra S., Knothe Tate M.L. (2003) Effect of lacunocanalicular architecture on hydraulic conductance in bone tissue: Implications for bone health and evolution, Anatomical Record, 273A(2): 752-762.

Steck R., Gatzka C., Schneider E., Niederer P., Knothe Tate M.L. (2003) Measurement of bone surface strains on the sheep metacarpus in vivo and ex vivo, Vet Comp Orthop Traumatol, 16(1): 38-43.

Steck R., Niederer P., Knothe Tate M.L. (2003) A finite element analysis for the prediction of load-induced fluid flow and mechanochemical transduction in bone. Journal of Theoretical Biology, 220(2): 249-259.

Tami A.E., Nasser P., Verborgt O., Schaffler M.B., Knothe Tate M.L. (2002) The role of interstitial fluid flow in the remodeling response to fatigue loading, Journal of Bone and Mineral Research, 17(11): 2030-2037.

Knothe Tate M.L., Tami A.E.G., Bauer T.W., Knothe U. (2002) Micropathoanatomy of Osteoporosis - Indications for a Cellular Basis of Bone Disease, Advances in Osteoporotic Fracture Management, 2(1): 9-14.

Knapp H.F., Reilly G.C., Stemmer A., Niederer P., Knothe Tate M.L. (2002) Development of preparation methods for and insights obtained from Atomic Force Microscopy of fluid spaces in cortical bone, Scanning, 24(1): 25-33.

Reilly G., Knapp H., Stemmer A., Niederer P., Knothe Tate M.L. (2001) Investigation of the morphology of the lacunocanalicular system of cortical bone using atomic force microscopy, Annals of Biomedical Engineering, 29(12): 1074-1081.

Niederer P.F., Knothe Tate M.L., Steck R., Boesiger P. (2000) Some remarks on intravascular and extravascular transport and flow dynamics, International Journal of Cardiovascular Medicine and Science, 3: 21-31.

Knothe Tate M.L., Steck R., Forwood M.R., Niederer P. (2000) In vivo demonstration of load-induced fluid flow in the rat tibia and its potential implications for processes associated with functional adaptation, Journal of Experimental Biology, 203(18): 2737-2745.

Knothe U., Knothe Tate M.L., Klaue K., Perren S.M. (2000) Development and testing of a new self-locking intramedullary nail system: Testing of handling aspects and mechanical properties, Injury, 31(8): 617-626.

Steck R., Niederer P., Knothe Tate M.L. (2000) A Finite Difference model of load-induced fluid displacements within bone under mechanical loading, Medical Engineering & Physics, 22: 117-125.

Gardner T.N., Stoll T., Marks L., Mishra S., Knothe Tate M.L. (2000) The influence of mechanical stimulus on the pattern of tissue differentiation in a long bone fracture – an FEM study, Journal of Biomechanics, 33(4): 415-425.

Knothe Tate M.L., Knothe U. (2000) An ex vivo model to study transport processes and fluid flow in loaded bone, Journal of Biomechanics, 33(2): 247-254.

Knothe U., Knothe Tate M.L., Perren S.M. (2000) 300 years of intramedullary fixation - from Aztec practice to standard treatment modality, European Journal of Trauma, 5: 217-225.

Gatzka C., Knothe U., Niederer P., Schneider E., Knothe Tate M.L. (1999) A novel ex vivo model for investigation of fluid displacements in bone after endoprosthesis implantation, Journal of Materials Science: Materials in Medicine, 10: 801-806.

Knothe Tate M.L., Niederer P., Knothe U. (1998) In vivo tracer transport through the lacunocanalicular system of rat bone in an environment devoid of mechanical loading, Bone 22(2): 107-117.

Knothe Tate M.L., Knothe U., Niederer P. (1998) Experimental elucidation of mechanical load-induced fluid flow and its role in bone metabolism and functional adaptation, American Journal of Medical Science 316(3): 189-195.

Knothe Tate M.L., Niederer P. (1998) A theoretical FE-based model developed to predict the relative contribution of convective and diffusive transport mechanisms for the maintenance of local equilibria within cortical bone. HTD – Vol. 362/BED – Vol. 40, Advances in Heat and Mass Transfer in Biotechnology, (Ed. S. Clegg) The American Society of Mechanical Engineers, 133-142.

Turner A.S., Cordey J.R., Nunamaker D.M., Tate M.L., McIff T., Perren S.M. (1990) In vitro strain patterns in the intact equine metacarpus and metatarsus following plate luting. Veterinary and Comparative Orthopaedics and Traumatology. 33: 6-11.
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