Hodaka Fujii Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, Japan
2 protocols
Antoine de Morree Stanford university
37 protocols

Clara Lubeseder-Martellato Technical University of Munich
7 protocols

Lee-Hwa Tai Universite de Sherbrooke
20 protocols

Masato Yano Kumamoto Health Science University
2 protocols

Reviewer
Toshitsugu Fujita
  • Faculty, Hirosaki University Graduate School of Medicine
Research focus
  • 2 Author merit
2 Protocols published
In vitro Engineered DNA-binding Molecule-mediated Chromatin Immunoprecipitation (in vitro enChIP) Using CRISPR Ribonucleoproteins in Combination with Next-generation Sequencing (in vitro enChIP-Seq) for the Identification of Chromosomal Interactions
Authors:  Toshitsugu Fujita and Hodaka Fujii, date: 11/20/2017, view: 8103, Q&A: 0
We have developed locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies consisting of insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated ChIP (enChIP). Locus-specific ChIP is a method to isolate a genomic ...
Identification of Proteins Interacting with Genomic Regions of Interest in vivo Using Engineered DNA-binding Molecule-mediated Chromatin Immunoprecipitation (enChIP)
Authors:  Toshitsugu Fujita and Hodaka Fujii, date: 05/20/2014, view: 13523, Q&A: 0
Elucidation of molecular mechanisms of genome functions requires identification of molecules interacting with genomic regions of interest in vivo. To this end, it is useful to isolate the target regions retaining molecular interactions. We ...
6 Protocols reviewed
Preparation of Human Chondrocytes for Profiling Using Cytometry by Time-of-flight (cyTOF)
Authors:  Fiorella Carla Grandi and Nidhi Bhutani, date: 07/20/2021, view: 2090, Q&A: 0

Single-cell technologies have allowed high-resolution profiling of tissues and thus a deeper understanding of tissue homeostasis and disease heterogeneity. Understanding this heterogeneity can be especially important for tailoring treatments in a

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Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
Authors:  Ryan R. Cupo and James Shorter, date: 12/05/2020, view: 2833, Q&A: 0

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the

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