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Overview
Authored
(1)
AH
Aylin S Haas
Ph.D candidate, Department of Developmental Physiology, Heidelberg University/ Centre for Organismal Studies
Research fields
-
Peer-reviewed
Preprint
BMV Propagation, Extraction and Purification Using Chromatographic Methods
Authors:
Aleksander Strugała
,
Paulina Bierwagen
,
Jakub Dalibor Rybka
,
Michał Giersig
,
Marek Figlerowicz
and
Anna Urbanowicz
,
date:
07/20/2018,
view:
5836,
Q&A:
0
Brome mosaic virus (BMV) is a well-known plant virus representing single-stranded RNA (ssRNA) positive-sense viruses. It has been widely used as a model in multiple studies concerning plant virus biology, epidemiology and the application of viral capsids in nanotechnology. Herein, we describe a method for BMV purification based on ion-exchange and size-exclusion chromatography. The presented method is of similar efficiency to previously described protocols relying on differential centrifugation and can easily be scaled up. The resulting BMV capsids are stable and monodisperse and can be used for further applications.
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