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Protocols
Biochemistry
Biophysics
Cancer Biology
Cell Biology
Developmental Biology
Immunology
Microbiology
Molecular Biology
Neuroscience
Plant Science
Stem Cell
Systems Biology
Articles and Issues
Current Issue
All Issues
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For Authors
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Submit a Protocol
Editorial Process
Editorial Criteria
AI-Generated Material
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Competing Interests
Article Processing Charges
About
About Us
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Overview
Authored
(1)
Q&A
(1)
HK
Harald Keller
INRA, Université Côte d’Azur, CNRS, Institut Sophia Agrobiotech, France, France,
Research fields
Plant science
Peer-reviewed
Preprint
In vitro
Microtubule Bundling Assay under Physiological Conditions
Authors:
Zach Geisterfer
,
Gin Tezuka
and
Li Tao
,
date:
04/05/2017,
view:
8523,
Q&A:
0
Kinesins play a role in organizing the mitotic spindle through the crosslinking of microtubules (MTs), made possible through binding sites at opposite ends of the holoenzyme. Here, we developed a method to test kinesin MT crosslinking action under physiological conditions.
More >
Gliding Assay to Analyze Microtubule-based Motor Protein Dynamics
Authors:
Albert Shim
,
Gin Tezuka
,
Luke Kupcha
and
Li Tao
,
date:
04/05/2017,
view:
13299,
Q&A:
0
The purpose of this protocol is to provide an updated method of performing microtubule gliding assays and visualizing it using fluorescence microscopy.
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