摘要:厌氧微生物生活在无氧的环境中,与需氧微生物相比培养技术特殊。本文介绍了厌氧微生物培养基制备方法,具体营养成分需要根据不同微生物的种类进行选择。
关键词: 厌氧微生物, 培养基, 培养
材料与试剂
仪器设备
实验步骤
一、厌氧微生物培养基配制
二、厌氧微生物培养
以瘤胃微生物培养为例 (Hailemariam et al., 2020)
致谢
感谢中国农业科学院创新工程 (ASTIP-IAS12) 支持。采用本实验方法发表的文章如下:Hailemariam, S., Zhao, S. and Wang, J. (2020). Complete genome sequencing and transcriptome analysis of nitrogen metabolism of Succinivibrio dextrinosolvens strain z6 isolated from dairy cow rumen. Front Microbiol 11: 1826.
参考文献
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