实验原理:水稻叶绿体是水稻光合作用和多种激素合成的场所,很多核编码蛋白及叶绿体编码蛋白在叶绿体中发挥重要功能。水稻叶绿体蛋白的分离需先用机械研磨叶片,进行percoll密度梯度离心,以分离出完整未破损叶绿体,再用细胞裂解液将叶绿体裂解,分离出叶绿体蛋白。实验目的:分离叶绿体蛋白,研究蛋白的定位、功能及质核信号的传导。
关键词: 水稻, 叶绿体蛋白, 分离检测
材料与试剂
仪器设备
实验步骤
注意事项
溶液配方
参考文献
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