Thank you for the protocol. I am having trouble detecting YAP after bacterial lysis. IPTG seems to induce a whole series of bands, and nothing obvious at full length YAP size, but the IPTG does have effect.
First, is the plasmid full length YAP? In the citation of the source they don't directly refer to that plasmid, and their main focus is on a truncated form.
Second, when you lyse the bacteria for rYAP, is there a reason for its being EDTA free?
Best,
Nathaniel
8/2/2018 9:46:44 AM Reply