KG
Kun‐Liang Guan
  • Department of Pharmacology , University of California San Diego, La Jolla
Research fields
  • Cancer biology
  • Peer-reviewed
  • Preprint
Non-radioactive LATS in vitro Kinase Assay
Authors:  Audrey W. Hong and Kun-Liang Guan, date: 07/20/2017, view: 11295, Q&A: 1
This protocol describes a method to directly measure LATS activity by an in vitro kinase assay using YAP as a substrate.
LDH-A Enzyme Assay
Authors:  Di Zhao, Yue Xiong, Qun-Ying Lei and Kun-Liang Guan, date: 10/05/2013, view: 14531, Q&A: 0
LDH (Lactate dehydrogenase) enzyme catalyzes the reversible conversion of pyruvate to lactate using NAD+ as a cofactor. Although the physiological significance of lactate accumulation in tumor cells, a dead-end product in cellular metabolism, is currently a topic of debate, it has long been known that many tumor cells express a high level of LDH-A (Koukourakis et al., 2003; Koukourakis et al., 2006; Koukourakis et al., 2009). So detection of its enzyme activity in vitro is important for researching on LDH-A. Recently, it has been reported that Lys-5 acetylation could decrease LDH-A enzyme activity (Zhao et al., 2013).
Staining and microscopy
Authors: Zhipeng Meng, Fu-Long Li and Kun-Liang Guan date: 2022-06-27 view: 572
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