Cancer Biology

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    Protocols in Current Issue
    Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter
    Authors:  Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang, date: 05/20/2020, view: 857, Q&A: 0
    [Abstract] In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN), and the clustered regularly interspaced short ...
    SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
    Authors:  Sunetra Roy and Katharina Schlacher, date: 09/20/2019, view: 1682, Q&A: 0
    [Abstract] The duplication of DNA is a fundamental process that is required for the transfer of the genetic information from parent to daughter cells. Aberrant DNA replication processes are associated with diverse disease phenotypes, including developmental defects, ageing disorders, blood disorders such as Fanconi Anemia, increased inflammation and cancer. ...
    DNA Fiber Assay upon Treatment with Ultraviolet Radiations
    Authors:  Alfano Luigi, Antonio Giordano and Francesca Pentimalli, date: 06/05/2017, view: 11265, Q&A: 0
    [Abstract] Genome stability is continuously challenged by a wide range of DNA damaging factors. To promote a correct DNA repair and cell survival, cells orchestrate a coordinated and finely tuned cascade of events collectively known as the DNA Damage Response (DDR). Ultra Violet (UV) rays are among the main environmental sources of DNA damage and a well ...
    DNA Damage Induction by Laser Microirradiation
    Authors:  Marianna Tampere and Oliver Mortusewicz, date: 12/05/2016, view: 13916, Q&A: 1
    [Abstract] Genome instability can lead to cell death, senescence and cancerous transformation. Specific repair pathways have evolved to prevent accumulation of DNA lesions. Studying these highly dynamic and specific repair pathways requires precise spatial and temporal resolution, which can be achieved through a combination of laser microirradiaiton and live ...
    Detection of Anaphase Bridge Formation by Immunofluorescence Microscopy in Mammalian Cells
    Authors:  Thomas Aschacher and Florian Enzmann, date: 10/20/2016, view: 7820, Q&A: 0
    [Abstract] The aim of this protocol is to provide a comprehensive description of the materials, equipment and reproducible methods to detect and analyze anaphase bridges in immunofluorescence microscopy using DAPI to detect cells that failed to completely segregate during mitosis. It describes the process of cell preparation, staining and microscopic ...
    Karyotype Analysis
    Authors:  Chun-Hung Chou and Muh-Hwa Yang, date: 05/20/2014, view: 20949, Q&A: 0
    [Abstract] A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to ...



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