Microbiology

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    Protocols in Current Issue
    In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.
    [Abstract] The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...
    Culturing Bacteria from Caenorhabditis elegans Gut to Assess Colonization Proficiency
    [Abstract] Determining an accurate count of intestinal bacteria from Caenorhabditis elegans is one critical way to assess colonization proficiency by a given bacteria. This can be accomplished by culturing appropriate dilutions of worm gut bacteria on selective or differential agarized media. Because of the high concentration of bacteria in gut ...
    Root-knot Nematode Penetration and Sclareol Nematicidal Activity Assays
    Authors:  Taketo Fujimoto, Takayuki Mizukubo, Hiroshi Abe and Shigemi Seo, date: 06/20/2016, view: 5457, Q&A: 0
    [Abstract] Plant parasitic nematodes parasitize roots and/or stems of various plants thereby inhibiting absorption of nutrients and moisture. In particular, root-knot nematodes (RKN) are a group of the most devastating pests. Various techniques, such as soil sterilization, cultivation of resistant crops, and chemical application, have been developed to ...
    Detection and Visualization of Specific Gene Transcripts by in situ RT-PCR in Nematode-Infected Arabidopsis Root Tissue
    Author:  Krzysztof Wieczorek, date: 09/20/2015, view: 5933, Q&A: 0
    [Abstract] This protocol describes an effective method of in situ RT-PCR that was developed to localize specific gene expression directly in thin cross-sections of nematode feeding sites induced by the cyst nematode Heterodera schachtii (H. schachtii) or the root-knot nematode Meloidogyne incognita (M. incognita) ...
    Infection Assay of Cyst Nematodes on Arabidopsis Roots
    Authors:  Holger Bohlmann and Krzysztof Wieczorek, date: 09/20/2015, view: 6832, Q&A: 0
    [Abstract] Plant parasitic nematodes are devastating pests on many crops. Juveniles (J2) of cyst nematodes invade the roots to induce a syncytium. This feeding site is their only source of nutrients. Male nematodes leave the roots after the fourth molt to mate with females. The females stay attached to their syncytia throughout their life and produce ...



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