Immunology

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    Protocols in Current Issue
    Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry
    Authors:  Michael D. Leipold and Holden Maecker, date: 01/20/2015, view: 13407, Q&A: 3
    [Abstract] Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques are being developed. In recent years, mass cytometry has emerged, wherein antibodies labeled with ...
    Intracellular Cytokine Staining on PBMCs Using CyTOFTM Mass Cytometry
    Authors:  Dongxia Lin, Sheena Gupta and Holden Maecker, date: 01/05/2015, view: 16704, Q&A: 2
    [Abstract] In this protocol, we use a CyTOFTM mass cytometry to collect single-cell data on a large number of cytokines/chemokines as well as cell-surface proteins that characterize T cells and other immune cells. The current selected mass window in AW 103-203 includes the lanthanides used for most antibody labeling, along with iridium and rhodium ...



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