Systems Biology

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    Protocols in Current Issue
    Cell Type-specific Metabolic Labeling of Proteins with Azidonorleucine in Drosophila
    [Abstract] Advanced mass spectrometry technology has pushed proteomic analyses to the forefront of biological and biomedical research. Limitations of proteomic approaches now often remain with sample preparations rather than with the sensitivity of protein detection. However, deciphering proteomes and their context-dependent dynamics in subgroups of ...
    Character-State Reconstruction to Infer Ancestral Protein-Protein Interaction Patterns
    Authors:  Florian Rümpler, Günter Theißen and Rainer Melzer, date: 08/20/2015, view: 7888, Q&A: 0
    [Abstract] Protein-protein interactions are at the core of a plethora of developmental, physiological and biochemical processes. Consequently, insights into the origin and evolutionary dynamics of protein-protein interactions may provide information on the constraints and dynamics of specific biomolecular circuits and their impact on the organismal ...
    Protein Sample Preparation for Proteomic Analysis in Leishmania donovani
    Authors:  Alexandros Alexandratos and Despina Smirlis, date: 03/05/2014, view: 7310, Q&A: 0
    [Abstract] Leishmania is a genus of trypanosomatid protozoa and is the parasite responsible for the disease leishmaniasis. These protozoa, regulate their gene expression in an atypical way, compared to other higher eukaryotes. The regulation of gene expression is characterized by a predominance of post-transcriptional over pre-transcriptional ...
    Proteolytic Fragment Isolation and Analysis (ex. N-terminal GST-tagged CLAVATA3 Protein GST-CLV3)
    Author:  Jun Ni, date: 07/20/2012, view: 8427, Q&A: 0
    [Abstract] It has become clear that the post-embryonic growth and development of plants requires properly controlled short distance cell-to-cell communication not only through the historically well-known phytohormones, but also through secreted small peptide signals. This protocol demonstrates an example of how to isolate small peptides (< 10 daltons) ...



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