Surgical Induction of Endometriosis in Female Mice
[Abstract] Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous ...
Isolation and Quantification of Extracellular DNA from Biofluids
[Abstract] Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties. Extracellular DNA can be extracted from plasma, urine, saliva or other biofluids using standard DNA isolation procedures and specialized commercial kits. Sample preparation for ...
Evaluation of B Cell Proliferation in vivo by EdU Incorporation Assay
Authors: Adi Biram and
Ziv Shulman,
date: 05/05/2020,
view: 1220,
Q&A: 0
[Abstract] Generation of antibodies is crucial for establishing enduring protection from invading pathogens, as well as for maintaining homeostasis with commensal bacteria at mucosal surfaces. Chronic exposure to microbiota- and dietary- derived antigens results in continuous production of antibody producing cells within the Peyer’s patch germinal center ...
Environmental Conditioning and Aerosol Infection of Mice
Authors: Eriko Kudo and
Akiko Iwasaki,
date: 04/20/2020,
view: 1246,
Q&A: 0
[Abstract] Influenza infection models in mice are widely used to study flu-mediated immune responses and pathology. However, most laboratory mice are housed at 20 °C and 50% relative humidity (RH). To better recapitulate influenza epidemics and immune responses during winter seasons, mice were housed at 20 °C under different humidity conditions, 10-20% or ...
Skin Transplantation and Lymphoid Organ Analysis in Mice
[Abstract] Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft retention. In this protocol, we provide a detailed procedure for skin grafting using the tail skin as ...