RNA Sequencing of Single Myofibers from Mus musculus
[Abstract] Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but this captures non-myogenic cells that are part of the muscle. In order to analyze only the ...
Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus
[Abstract] The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular environment. This biofluid contains an enriched source of proteins, ions, and other signaling molecules for ...
Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation
Authors: Gileade P. Freitas,
Alann T. P. Souza,
Helena B. Lopes,
Rayana L. B. Trevisan,
Fabiola S. Oliveira,
Roger R. Fernandes,
Fernanda U. Ferreira,
Felipe A. Ros,
Marcio M. Beloti and
Adalberto L. Rosa,
date: 02/20/2020,
view: 55,
Q&A: 0
[Abstract] Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and ...
In vitro Self-organized Mouse Small Intestinal Epithelial Monolayer Protocol
[Abstract] Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed culture models showed physiological-relevant cell composition but did not prove self-renewing ...
Mouse Embryonic Tooth Germ Dissection and Ex vivo Culture Protocol
[Abstract] A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and placed on cell culture inserts for observation of subsequent tooth germ development in a ...
Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels
Authors: Tzemach Aouizerat,
Aren M. Maeir,
Yitzhak Paz,
Yuval Gadot,
Amir Szitenberg,
Sivan Alkalay-Oren,
Shunit Coppenhagen-Glazer,
Michael Klutstein and
Ronen Hazan,
date: 01/05/2020,
view: 592,
Q&A: 0
[Abstract] Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that ...