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David Paul
MRC Laboratory of Molecular Biology
47 protocols
Thirupugal Govindarajan
Cincinnati Children's Hospital Medical Center
13 protocols
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Reviewer
Sylvain Baron
Cellecta
Research focus
Cancer biology
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2 Protocols reviewed
Screening Method for CRISPR/Cas9 Inhibition of a Human DNA Virus: Herpes Simplex Virus
Authors:
Werner M. Neuhausser
,
Hyung S. Oh
,
Pierce Eggan
,
Magdalena Angelova
,
Rory Kirchner
,
Kevin C. Eggan
and
David M. Knipe
,
date:
09/05/2020,
view:
4064,
Q&A:
0
The efficiency of cleavage of individual CRISPR/Cas9-sgRNAs remains difficult to predict based on the CRISPR target sequence alone. Different intracellular environments (dependent on cell type or cell cycle state for example) may affect sgRNA ...
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CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
Authors:
Naoaki Mizuno
,
Eiji Mizutani
,
Hideyuki Sato
,
Mariko Kasai
,
Hiromitsu Nakauchi
and
Tomoyuki Yamaguchi
,
date:
07/05/2019,
view:
4549,
Q&A:
0
Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA. Viral vectors are ...
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