Aims
RNAs are biomolecules crucial for gene expression and protein synthesis, and the amount, timing, and location of their synthesis and activity are key for cellular function and identity. Recent advances in RNA detection have allowed scientists to move from traditional methods like Northern blotting and RT-qPCR to higher spatial or temporal resolution techniques, such as single-cell nascent RNA sequencing and fluorescently labeled aptamers for single-molecule mRNA imaging in living cells. These developments are paving the way for new, exciting pathways toward understanding complex RNA-based biological processes.
Scope
This special issue aims to highlight the latest technological advancements in RNA detection, including but not limited to:
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RNA sequencing, e.g., single-cell RNAseq, single-nuclei RNAseq, in situ RNAseq techniques, long-read sequencing for RNA isoforms and modifications, and SMRT-RNAseq using Nanopore or microfluidic platforms
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RNA visualization, e.g., fluorescence in situ hybridization (FISH)-based techniques (e.g., seqFISH, smFISH, ExFISH), and RNA-binding aptamer- or CRISPR-fluorophore systems
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Enhanced chemistry for RNA detection, e.g., bioorthogonal click chemistry or proximity ligation assays for RNA
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Bioinformatics tools, e.g., analysis pipelines for RNAseq data or RNA structure and function predictions
Submission guidelines
Special issue editors
Kif Liakath-Ali, Ph.D., Features Editor,
kif.liakath-ali@soton.ac.uk
Lecturer, University of Southampton
Marion Hogg, Ph.D., Associate Editor,
marion.hogg@ntu.ac.uk
Senior Lecturer, Nottingham Trent University
Renate Weizbauer, Ph.D., Managing Editor,
rweizbauer@ed.bio-protocol.org
Post-Doc, Carnegie Institution for Science
For any inquiries regarding this special issue, please contact the editors at the email addresses provided above.
About the Bio-protocol journal
Established in 2011 by a group of postdoctoral researchers at Stanford University,
Bio-protocol is a peer-reviewed, open-access protocol journal. Its mission is to make life science research more reproducible by publishing high-quality, step-by-step protocols.
Bio-protocol has published over 5,000 protocols authored by more than 20,000 scientists worldwide.
Bio-protocol editorial and reviewing boards consist mostly of postdocs and junior faculty members. These early-career life science researchers, who actively and routinely design, conduct, and supervise lab experiments, are well-positioned to assess the quality and clarity of protocols.
Bio-protocol articles are listed in PubMed Central and indexed in Web of Science (ESCI) and DOAJ.
Bio-protocol has built tight collaborations with several high-profile research journals (e.g.,
eLife &
Journal of Biological Chemistry) to advance our mutual goal of making scientific literature more reproducible.