Nicoletta Cordani —BIO-PROTOCOL

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Clara Sciorati Division of Regenerative Medicine, Ospedale San Raffaele, Italy
1 protocol

Laura Pozzi Scientific Institute, Italy
1 protocol

Viviana Pisa Department of Biomedical and Clinical Sciences, University of Milano, Italy
1 protocol

Abhijit Kale Buck Institute for Research on Aging
5 protocols

Agnieszka Pastula Columbia University in the City of New York
4 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
12 protocols

Antoine de Morree Stanford university
33 protocols

Editor

Nicoletta Cordani

Post-Doc, University of Milano-Bicocca

Research focus

Cancer biology

1 Author merit
Reviewer merit
Editor merit

Lab information

University of Milano-Bicocca

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=cordani+n

1 Protocol published

Isolation of FAP Cells from Mouse Dystrophic Skeletal Muscle Using Fluorescence Activated Cell Sorting

Authors: Nicoletta Cordani, Viviana Pisa, Laura Pozzi and Clara Sciorati, date: 11/20/2014, view: 8150, Q&A: 1

A population of muscle resident CD45^-, CD31^- cells expressing the mesenchymal PDGF receptor alpha (PDGFRα) as well as Sca-1 was first isolated in healthy mouse muscles in Uezumi et al. (2010). In the same year, Joe et ...

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Huan Li

Sun Yat-sen University

I want to know the size of FAP cells since the information is important for my experiment. I am looking forward to the answer. Thank you!

5/11/2018 9:43:50 PM Reply

Huan Li

Sun Yat-sen University

Thank you for your answer! It is helpful.

2018-05-27 23:46:28

Clara Sciorati

Division of Regenerative Medicine, Ospedale San Raffaele, Italy

Hi
what do you mean for size? they are quite similar to satellite cells...

2018-05-27 23:39:16

Clara Sciorati

Division of Regenerative Medicine, Ospedale San Raffaele, Italy

In plate FAP cells are around 20-25um.
All my best and cross the finger for your experiment!

2018-05-27 12:25:52

3 Protocols reviewed

Differentiation of Human Embryonic Stem Cells into Cone Photoreceptors

Authors: Anthony Flamier, Andrea Barabino and Gilbert Bernier, date: 07/20/2016, view: 5035, Q&A: 1

Photoreceptors are specialized retinal neurons able to respond to light in order to generate visual information. Among photoreceptors, cones are involved in colors discrimination and high-resolution central vision and are selectively depleted in ...

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Laser Microirradiation and Temporal Analysis of XRCC1 Recruitment to Single-strand DNA Breaks

Authors: Anna Campalans and J. Pablo Radicella, date: 03/05/2016, view: 4962, Q&A: 0

The DNA molecule is exposed to a multitude of damaging agents that can compromise its integrity: single (SSB) and double strand breaks (DSB), intra- or inter-strand crosslinks, base loss or modification, etc. Many different DNA repair ...

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19 Protocols edited

A Flow Cytometric Method to Determine Transfection Efficiency

Authors: Wenli Mu, Stefanie Homann, Christian Hofmann, Alexandr Gorin, Diana Huynh, Otto Orlean Yang and Theodoros Kelesidis, date: 05/20/2019, view: 548, Q&A: 0

Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function. Although several transfection strategies have been developed, there is a wide ...

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A Functionally Robust Phenotypic Screen that Identifies Drug Resistance-associated Genes Using 3D Cell Culture

Authors: Sun-Young Lee and Mina J. Bissell, date: 11/20/2018, view: 1457, Q&A: 0

Drug resistance is a major obstacle in cancer treatment: A case in point is the failure of cancer patients to respond to tyrosine kinase inhibitors (TKI) of EGFR, a receptor that is highly expressed in many cancers. Identification of the targets and ...

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