Samuel Wilson Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
3 protocols

Julie Horton Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
1 protocol
Chong He Buck Institute for Research on Aging
7 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
71 protocols

Gregory Finnigan Kansas State University
2 protocols

Ralph Boettcher Max Planck Institute for Biochemistry
39 protocols

Melike Çağlayan
  • Assist. Prof., University of Florida
Research focus
  • Biochemistry
  • 3 Author merit



Lab information

Caglayan Lab

1) Caglayan M. and Wilson S.H. Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals a promutagenic DNA intermediate during double strand break repair. Nature Communications (2018) 9: 4213.
2) Horton J.K., Stefanick D.F., Caglayan M., Zhao M.L., Gassman N.R., Wilson S.H. XRCC1 phosphorylation affects aprataxin recruitment and DNA deadenylation activity. DNA Repair (2018) – 64: 26 – 33.
3) Prasad R., Caglayan M., Da-Peng D., Nadalutti C.A., Gassman N.R., Zhao M., Stefanick D.F., Horton J.K., Krasich R., Longley M.J., Copeland W.C., Griffith J.D., Wilson S.H. DNA polymerase β: The missing link of the base excision repair machinery in mammalian mitochondria. DNA Repair (2017) 60: 77 – 88.
4) Caglayan M., Prasad R., Krasich R., Longley M.J., Kadoda K., Tsuda M., Sasanuma H., Takeda S., Tano K., Copeland W.C., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes in mitochondrial extracts. Nucleic Acids Research (2017) 17: 10079 – 10088.
5) Caglayan M., Wilson S.H. Role of DNA polymerase β oxidized nucleotide insertion in DNA ligation failure. Journal of Radiation Research - Review article (2017) 1093: 1 – 5.
6) Caglayan M., Horton J.K., Da-Peng D., Stefanick D.F., Wilson S.H. Oxidized nucleotide insertion by pol β confounds ligation during base excision repair. Nature Communications (2017) 8: 14045.
7) Sassa A., Caglayan M., Rodriguez Y., Beard W.A., Wilson S.H., Nohmi T., Honma M., Yasui M. Impact of ribonucleotide backbone on translesion synthesis and repair of 7,8-Dihydro-8-oxoguanine. Journal of Biological Chemistry (2016) 291: 24314 – 24323.
8) Caglayan M., Wilson S.H. Oxidant and environmental toxicant-induced effects compromise DNA ligation during base excision DNA repair. DNA Repair - Review article (2015) 35: 85 – 89.
9) Caglayan M., Horton J.K., Prasad R., Wilson S.H. Complementation of aprataxin deficiency by base excision repair enzymes. Nucleic Acids Research (2015) 43: 2271 – 2281.
10) Caglayan M., Batra V.K., Sassa A., Prasad R., Wilson S.H. Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair. Nature Structural and Molecular Biology (2014) 21: 497 – 499.
11) Sassa A., Caglayan M., Dyrkheeva N.S., Beard W.A., Wilson S.H. Base excision repair of tandem modifications in a methylated CpG dinucleotide. Journal of Biological Chemistry (2014) 289: 13996 – 4008.
3 Protocols published
In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
Authors:  Melike Çağlayan and Samuel H. Wilson, date: 06/20/2017, view: 4465, Q&A: 0
We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide ...
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells
Authors:  Melike Çağlayan, Julie K. Horton and Samuel H. Wilson, date: 06/05/2015, view: 5202, Q&A: 0
We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, ...
3 Protocols reviewed
RNA Capping by Transcription Initiation with Non-canonical Initiating Nucleotides (NCINs): Determination of Relative Efficiencies of Transcription Initiation with NCINs and NTPs
Authors:  Jeremy G. Bird, Bryce E. Nickels and Richard H. Ebright, date: 06/20/2017, view: 4593, Q&A: 0
It recently has been established that adenine-containing cofactors, including nicotinamide adenine dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), and 3’-desphospho-coenzyme A (dpCoA), can serve as ‘non-canonical ...
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Uptake Assay for Radiolabeled Peptides in Yeast
Authors:  Melinda Hauser, Houjian Cai, Fred Naider and Jeffrey M. Becker, date: 11/20/2016, view: 4757, Q&A: 0
We describe an assay for measuring the uptake of radioactive peptides into the yeast Saccharomyces cerevisiae. The methods presented here can be adapted to measure a variety of substrates transported into any bacterial or fungal cell via ...
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