Antoine de Morree Stanford university
33 protocols

Fanglian He Bio-protocol
205 protocols

Lin Fang Stanford University
95 protocols

Nicoletta Cordani University of Milano-Bicocca
21 protocols

Editor
Hui Zhu
  • Stanford University
Research focus
  • Cell biology
  • 9 Author merit
Lab Protocol Hub
9 Protocols published
Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
Author:  Hui Zhu, date: 04/05/2012, view: 18210, Q&A: 3
Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell ...
Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
Author:  Hui Zhu, date: 04/05/2012, view: 52003, Q&A: 2
Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
17 Protocols reviewed
LT-HSC Methylcellulose Assay
Author:  Marc A. Kerenyi, date: 03/05/2014, view: 11557, Q&A: 0
Hematopoietic differentiation is a highly complex process originating from an extraordinary population of cells called long-term repopulating hematopoietic stem cells (LT-HSCs). The unique feature of all stem cells, including HSCs, is their ...
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X-gal Staining on Adult Mouse Brain Sections
Authors:  Hiroshi Kokubu and Janghoo Lim, date: 03/05/2014, view: 10934, Q&A: 0
Knowing expression patterns of given proteins is very important to understand their functions. Immunostaining analysis with specific antibodies is commonly used to identify cells or tissues expressing proteins of interest. Although this technique ...
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